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Assay Detail

CHEMBL4727121

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Mixed inhibition of recombinant human AChE expressed in HEK293 cells assessed as dissociation constant for enzyme-substrate-inhibitor complex using varying levels of acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition by Line-weaver Burk plot analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Centrally Active Multitarget Anti-Alzheimer Agents Derived from the Antioxidant Lead CR-6.
Pérez-Areales FJ,Garrido M,Aso E,Bartolini M,De Simone A,Espargaró A,Ginex T,Sabate R,Pérez B,Andrisano V,Puigoriol-Illamola D,Pallàs M,Luque FJ,Loza MI,Brea J,Ferrer I,Ciruela F,Messeguer A,Muñoz-Torrero D
J Med Chem (2020) 63 : 9360 -9390
PubMed 32706255 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 9.59 9.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4751100 1 9.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4751100 Ki = 0.258 nM 9.59