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Assay Detail

CHEMBL4728539

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant mouse soluble epoxide hydrolase using CMNPC as substrate preincubated for 5 mins followed by substrate addition and measured at 30 secs interval for 10 mins by fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bifunctional epoxide hydrolase 2 (CHEMBL4140)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

2-Oxaadamant-1-yl Ureas as Soluble Epoxide Hydrolase Inhibitors: In Vivo Evaluation in a Murine Model of Acute Pancreatitis.
Codony S,Pujol E,Pizarro J,Feixas F,Valverde E,Loza MI,Brea JM,Saez E,Oyarzabal J,Pineda-Lucena A,Pérez B,Pérez C,Rodríguez-Franco MI,Leiva R,Osuna S,Morisseau C,Hammock BD,Vázquez-Carrera M,Vázquez S
J Med Chem (2020) 63 : 9237 -9257
PubMed 32787085 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.41 7.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4784603 1 7.12
CHEMBL4784283 1 7.10
CHEMBL4759592 1 7.04
CHEMBL4753341 1 6.97
CHEMBL4800149 1 6.89
CHEMBL4758083 1 6.76
CHEMBL4764423 1 6.63
CHEMBL4785423 1 6.54
CHEMBL4778324 1 6.46
CHEMBL4746168 1 6.44
CHEMBL4762846 1 6.29
CHEMBL4797443 1 6.26
CHEMBL4750570 1 6.14
CHEMBL4762265 1 5.73
CHEMBL4790388 1 5.41
CHEMBL4764141 1 4.83
CHEMBL4748933 1 -
CHEMBL4751517 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4784603 IC50 = 75.9 nM 7.12
CHEMBL4784283 IC50 = 79.1 nM 7.10
CHEMBL4759592 IC50 = 92.3 nM 7.04
CHEMBL4753341 IC50 = 107.0 nM 6.97
CHEMBL4800149 IC50 = 130.0 nM 6.89
CHEMBL4758083 IC50 = 174.0 nM 6.76
CHEMBL4764423 IC50 = 234.0 nM 6.63
CHEMBL4785423 IC50 = 291.0 nM 6.54
CHEMBL4778324 IC50 = 344.0 nM 6.46
CHEMBL4746168 IC50 = 365.0 nM 6.44
CHEMBL4762846 IC50 = 512.0 nM 6.29
CHEMBL4797443 IC50 = 553.0 nM 6.26
CHEMBL4750570 IC50 = 723.0 nM 6.14
CHEMBL4762265 IC50 = 1857.0 nM 5.73
CHEMBL4790388 IC50 = 3853.0 nM 5.41
CHEMBL4764141 IC50 = 14721.0 nM 4.83
CHEMBL4748933 IC50 - -
CHEMBL4751517 IC50 > 100.0 nM -