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Assay Detail

CHEMBL4730561

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant EGFR expressed in baculovirus infected Sf9 insect cells using poly (Glu, Tyr) 4:1 peptide as substrate incubated for 40 mins in presence of 10 uM ATP by ADP-Glo luminescence assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of a Potent Brain-Penetrant EGFR Tyrosine Kinase Inhibitor against Malignant Brain Tumors.
Tsang JE,Urner LM,Kim G,Chow K,Baufeld L,Faull K,Cloughesy TF,Clark PM,Jung ME,Nathanson DA
ACS Med Chem Lett (2020) 11 : 1799 -1809
PubMed 33062157 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.75 8.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL553 ERLOTINIB 4.0 1 8.66
CHEMBL4743220 1 8.60
CHEMBL4744655 1 8.41
CHEMBL4783428 1 8.21
CHEMBL4740063 1 8.19
CHEMBL4791756 1 8.12
CHEMBL4786961 1 8.00
CHEMBL4759031 1 7.98
CHEMBL1198370 1 7.87
CHEMBL4793453 1 7.81
CHEMBL4762453 1 7.79
CHEMBL4749501 1 7.72
CHEMBL4750375 1 7.71
CHEMBL4794223 1 7.68
CHEMBL4743464 1 7.66
CHEMBL4778159 1 7.62
CHEMBL4744207 1 7.60
CHEMBL4759650 1 7.38
CHEMBL4453068 1 7.31
CHEMBL4786797 1 6.30
CHEMBL4793944 1 6.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL553 ERLOTINIB IC50 = 2.17 nM 8.66
CHEMBL4743220 IC50 = 2.49 nM 8.60
CHEMBL4744655 IC50 = 3.91 nM 8.41
CHEMBL4783428 IC50 = 6.16 nM 8.21
CHEMBL4740063 IC50 = 6.41 nM 8.19
CHEMBL4791756 IC50 = 7.63 nM 8.12
CHEMBL4786961 IC50 = 10.0 nM 8.00
CHEMBL4759031 IC50 = 10.4 nM 7.98
CHEMBL1198370 IC50 = 13.6 nM 7.87
CHEMBL4793453 IC50 = 15.6 nM 7.81
CHEMBL4762453 IC50 = 16.2 nM 7.79
CHEMBL4749501 IC50 = 18.9 nM 7.72
CHEMBL4750375 IC50 = 19.7 nM 7.71
CHEMBL4794223 IC50 = 21.0 nM 7.68
CHEMBL4743464 IC50 = 22.0 nM 7.66
CHEMBL4778159 IC50 = 24.0 nM 7.62
CHEMBL4744207 IC50 = 25.0 nM 7.60
CHEMBL4759650 IC50 = 41.4 nM 7.38
CHEMBL4453068 IC50 = 48.7 nM 7.31
CHEMBL4786797 IC50 = 505.1 nM 6.30
CHEMBL4793944 IC50 = 782.8 nM 6.11