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Assay Detail

CHEMBL4731276

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of URAT1 in human RPTEC assessed as inhibition of [14C]uric acid uptake preincubated for 15 mins followed by [14C]uric acid addition and incubated under shaking condition for 2 hrs by scintillation counter method
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type RPTEC
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Solute carrier family 22 member 12 (CHEMBL6120)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Dotinurad (FYU-981), a New Phenol Derivative with Highly Potent Uric Acid Lowering Activity.
Uda J,Kobashi S,Miyata S,Ashizawa N,Matsumoto K,Iwanaga T
ACS Med Chem Lett (2020) 11 : 2017 -2023
PubMed 33062187 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 4.97 5.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4762461 1 5.60
CHEMBL4748804 1 5.58
CHEMBL4749075 1 5.50
CHEMBL4792097 1 5.37
CHEMBL4796932 1 5.28
CHEMBL4760971 1 5.24
CHEMBL388590 BENZBROMARONE 4.0 1 5.17
CHEMBL4594446 DOTINURAD 3.0 1 5.17
CHEMBL4756915 1 5.15
CHEMBL4796568 1 5.06
CHEMBL4781629 1 4.97
CHEMBL4744184 1 4.88
CHEMBL4744854 1 4.83
CHEMBL4762003 1 4.75
CHEMBL4740995 1 4.72
CHEMBL4753182 1 4.50
CHEMBL4778323 1 4.47
CHEMBL4781818 1 4.08
CHEMBL4757464 1 4.08
CHEMBL4756625 1 -
CHEMBL4785815 1 -
CHEMBL4800504 1 -
CHEMBL4790389 1 -
CHEMBL4785752 1 -
CHEMBL4782683 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4762461 IC50 = 2500.0 nM 5.60
CHEMBL4748804 IC50 = 2600.0 nM 5.58
CHEMBL4749075 IC50 = 3200.0 nM 5.50
CHEMBL4792097 IC50 = 4300.0 nM 5.37
CHEMBL4796932 IC50 = 5300.0 nM 5.28
CHEMBL4760971 IC50 = 5700.0 nM 5.24
CHEMBL388590 BENZBROMARONE IC50 = 6800.0 nM 5.17
CHEMBL4594446 DOTINURAD IC50 = 6800.0 nM 5.17
CHEMBL4756915 IC50 = 7100.0 nM 5.15
CHEMBL4796568 IC50 = 8800.0 nM 5.06
CHEMBL4781629 IC50 = 10800.0 nM 4.97
CHEMBL4744184 IC50 = 13100.0 nM 4.88
CHEMBL4744854 IC50 = 14700.0 nM 4.83
CHEMBL4762003 IC50 = 17800.0 nM 4.75
CHEMBL4740995 IC50 = 19200.0 nM 4.72
CHEMBL4753182 IC50 = 31600.0 nM 4.50
CHEMBL4778323 IC50 = 33900.0 nM 4.47
CHEMBL4781818 IC50 = 83300.0 nM 4.08
CHEMBL4757464 IC50 = 83900.0 nM 4.08
CHEMBL4756625 IC50 > 100000.0 nM -
CHEMBL4785815 IC50 > 100000.0 nM -
CHEMBL4800504 IC50 > 100000.0 nM -
CHEMBL4790389 IC50 > 100000.0 nM -
CHEMBL4785752 IC50 > 100000.0 nM -
CHEMBL4782683 IC50 > 100000.0 nM -