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Assay Detail

CHEMBL4731452

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD4 bromodomain1 (unknown origin) using peptide histone H4 (SGRGACKGGACKGLGAC-KGGAACKRHGSGSK-biotin) as substrate incubated for 15 mins followed by substrate addition measured after 1 hr by alphascreen assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of 3,5-Dimethylisoxazole Derivatives as BRD4 Inhibitors for the Treatment of Colorectal Cancer.
Yang Y,Fang L,Chen P,Zhang H,Zhou J
ACS Med Chem Lett (2020) 11 : 2174 -2181
PubMed 33214826 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.77 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3987016 MIVEBRESIB 2.0 1 8.60
CHEMBL4793308 1 8.38
CHEMBL4778807 1 8.11
CHEMBL4795650 1 7.89
CHEMBL4781744 1 7.72
CHEMBL4764687 1 7.51
CHEMBL4637198 1 7.50
CHEMBL4743695 1 7.38
CHEMBL1957266 1 7.38
CHEMBL4751282 1 7.25
CHEMBL4790523 1 -
CHEMBL4799831 1 -
CHEMBL4758215 1 -
CHEMBL4749258 1 -
CHEMBL4788655 1 -
CHEMBL4756710 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3987016 MIVEBRESIB IC50 = 2.5 nM 8.60
CHEMBL4793308 IC50 = 4.2 nM 8.38
CHEMBL4778807 IC50 = 7.7 nM 8.11
CHEMBL4795650 IC50 = 13.0 nM 7.89
CHEMBL4781744 IC50 = 19.0 nM 7.72
CHEMBL4764687 IC50 = 31.0 nM 7.51
CHEMBL4637198 IC50 = 32.0 nM 7.50
CHEMBL4743695 IC50 = 42.0 nM 7.38
CHEMBL1957266 IC50 = 42.0 nM 7.38
CHEMBL4751282 IC50 = 56.0 nM 7.25
CHEMBL4790523 IC50 - -
CHEMBL4799831 IC50 < 2.1 nM -
CHEMBL4758215 IC50 < 2.1 nM -
CHEMBL4749258 IC50 < 2.1 nM -
CHEMBL4788655 IC50 < 2.1 nM -
CHEMBL4756710 IC50 < 2.1 nM -