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Assay Detail

CHEMBL4731980

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of fluorescent labelled fluormone ES2 from human recombinant GST tagged ER alpha LBD ( 282 to 595 residues) expressed in baculovirus infected insect cells incubate for 20 mins measured after 1 hr by TR-FRET Lanthascreen assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Addition of Fluorine and a Late-Stage Functionalization (LSF) of the Oral SERD AZD9833.
Scott JS,Moss TA,Barlaam B,Davey PRJ,Fairley G,Gangl ET,Greenwood RDR,Hatoum-Mokdad H,Lister AS,Longmire D,Polanski R,Stokes S,Tucker MJ,Varnes JG,Yang B
ACS Med Chem Lett (2020) 11 : 2519 -2525
PubMed 33335676 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 8.38 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4789498 1 9.30
CHEMBL4777139 1 9.00
CHEMBL4754652 1 8.90
CHEMBL4749445 1 8.80
CHEMBL4789223 1 8.70
CHEMBL4799234 1 8.70
CHEMBL4650365 CAMIZESTRANT 3.0 1 8.60
CHEMBL4780699 1 8.60
CHEMBL4754747 1 8.40
CHEMBL4745941 1 7.80
CHEMBL4746365 1 7.40
CHEMBL4757584 1 6.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4789498 IC50 = 0.5012 nM 9.30
CHEMBL4777139 IC50 = 1.0 nM 9.00
CHEMBL4754652 IC50 = 1.259 nM 8.90
CHEMBL4749445 IC50 = 1.585 nM 8.80
CHEMBL4789223 IC50 = 1.995 nM 8.70
CHEMBL4799234 IC50 = 1.995 nM 8.70
CHEMBL4650365 CAMIZESTRANT IC50 = 2.512 nM 8.60
CHEMBL4780699 IC50 = 2.512 nM 8.60
CHEMBL4754747 IC50 = 3.981 nM 8.40
CHEMBL4745941 IC50 = 15.85 nM 7.80
CHEMBL4746365 IC50 = 39.81 nM 7.40
CHEMBL4757584 IC50 = 501.19 nM 6.30