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Assay Detail

CHEMBL4733831

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PI3Kalpha using PIP2 as substrate in presence of ATP measured after 30 mins by HTRF assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Identification of methyl (5-(6-((4-(methylsulfonyl)piperazin-1-yl)methyl)-4-morpholinopyrrolo[2,1-f][1,2,4]triazin-2-yl)-4-(trifluoromethyl)pyridin-2-yl)carbamate (CYH33) as an orally bioavailable, highly potent, PI3K alpha inhibitor for the treatment of advanced solid tumors.
Xiang HY,Wang X,Chen YH,Zhang X,Tan C,Wang Y,Su Y,Gao ZW,Chen XY,Xiong B,Gao ZB,Chen Y,Ding J,Meng LH,Yang CH
Eur J Med Chem (2021) 209 : 112913 -112913
PubMed 33109399 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.44 8.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4800252 RISOVALISIB 2.0 1 8.23
CHEMBL2396661 ALPELISIB 4.0 1 7.75
CHEMBL4783228 1 7.75
CHEMBL4740055 1 7.70
CHEMBL4779801 1 7.68
CHEMBL4784118 1 7.63
CHEMBL4742751 1 7.40
CHEMBL4756324 1 7.39
CHEMBL4781518 1 7.38
CHEMBL4760391 1 7.35
CHEMBL4791280 1 7.30
CHEMBL4761702 1 7.26
CHEMBL4800181 1 7.17
CHEMBL4756074 1 6.98
CHEMBL4799728 1 6.61

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4800252 RISOVALISIB IC50 = 5.9 nM 8.23
CHEMBL2396661 ALPELISIB IC50 = 17.8 nM 7.75
CHEMBL4783228 IC50 = 17.7 nM 7.75
CHEMBL4740055 IC50 = 19.9 nM 7.70
CHEMBL4779801 IC50 = 21.0 nM 7.68
CHEMBL4784118 IC50 = 23.6 nM 7.63
CHEMBL4742751 IC50 = 39.6 nM 7.40
CHEMBL4756324 IC50 = 41.1 nM 7.39
CHEMBL4781518 IC50 = 41.7 nM 7.38
CHEMBL4760391 IC50 = 44.4 nM 7.35
CHEMBL4791280 IC50 = 50.5 nM 7.30
CHEMBL4761702 IC50 = 55.4 nM 7.26
CHEMBL4800181 IC50 = 67.5 nM 7.17
CHEMBL4756074 IC50 = 105.3 nM 6.98
CHEMBL4799728 IC50 = 243.8 nM 6.61