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Assay Detail

CHEMBL4734922

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]UR-PI294 from human SP-FLAG-tagged H3R expressed in HEK293T cells measured after 60 to 120 mins by liquid scintillation counting analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural modifications in the distal, regulatory region of histamine H receptor antagonists leading to the identification of a potent anti-obesity agent.
Szczepańska K,Pockes S,Podlewska S,Höring C,Mika K,Latacz G,Bednarski M,Siwek A,Karcz T,Nagl M,Bresinsky M,Mönnich D,Seibel U,Kuder KJ,Kotańska M,Stark H,Elz S,Kieć-Kononowicz K
Eur J Med Chem (2021) 213 : 113041 -113041
PubMed 33261900 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 7.66 8.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4783744 1 8.50
CHEMBL4741145 1 8.23
CHEMBL4776141 1 8.11
CHEMBL4743583 1 8.11
CHEMBL4758080 1 8.07
CHEMBL4786308 1 7.75
CHEMBL4800672 1 7.69
CHEMBL4750996 1 7.64
CHEMBL14638 CIPROXIFAN 1 7.31
CHEMBL4781093 1 7.22
CHEMBL4744110 1 7.17
CHEMBL4740135 1 6.96
CHEMBL4783066 1 6.86

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4783744 Ki = 3.17 nM 8.50
CHEMBL4741145 Ki = 5.84 nM 8.23
CHEMBL4776141 Ki = 7.7 nM 8.11
CHEMBL4743583 Ki = 7.86 nM 8.11
CHEMBL4758080 Ki = 8.43 nM 8.07
CHEMBL4786308 Ki = 17.63 nM 7.75
CHEMBL4800672 Ki = 20.28 nM 7.69
CHEMBL4750996 Ki = 22.98 nM 7.64
CHEMBL14638 CIPROXIFAN Ki = 48.9 nM 7.31
CHEMBL4781093 Ki = 60.67 nM 7.22
CHEMBL4744110 Ki = 67.19 nM 7.17
CHEMBL4740135 Ki = 109.06 nM 6.96
CHEMBL4783066 Ki = 139.74 nM 6.86