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Assay Detail

CHEMBL4735374

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full-length N-terminal GST-fused MNK2 (1 to 465 residues) expressed in baculovirus expression system using GRSRSRSRSR as substrate measured after 2 hrs by ADP-glo luminescence assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of indazole-pyridinone derivatives as a novel class of potent and selective MNK1/2 kinase inhibitors that protecting against endotoxin-induced septic shock.
Dreas A,Kucwaj-Brysz K,Pyziak K,Kulesza U,Wincza E,Fabritius CH,Michalik K,Gabor-Worwa E,Gołas A,Milik M,Masiejczyk M,Majewska E,Pyśniak K,Wójcik-Trechcińska U,Sandowska-Markiewicz Z,Brzózka K,Ostrowski J,Rzymski T,Mikula M
Eur J Med Chem (2021) 213 : 113057 -113057
PubMed 33303237 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 7.33 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4752141 1 8.40
CHEMBL4787269 1 8.30
CHEMBL4764756 1 8.22
CHEMBL4785028 1 8.10
CHEMBL4743561 1 7.92
CHEMBL4757431 1 7.68
CHEMBL4755524 1 7.50
CHEMBL4746396 1 7.50
CHEMBL4795993 1 7.43
CHEMBL4791136 1 7.42
CHEMBL4749973 1 7.40
CHEMBL4750622 1 7.37
CHEMBL4789590 1 7.12
CHEMBL4752921 1 7.11
CHEMBL4759018 1 7.08
CHEMBL4762761 1 7.01
CHEMBL4752471 1 7.00
CHEMBL4790632 1 6.97
CHEMBL4763087 1 6.47
CHEMBL4793486 1 5.97
CHEMBL4776712 1 5.95
CHEMBL4793538 1 -
CHEMBL4746326 1 -
CHEMBL4752505 1 -
CHEMBL4765071 1 -
CHEMBL4758288 1 -
CHEMBL4757267 1 -
CHEMBL4763764 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4752141 IC50 = 4.0 nM 8.40
CHEMBL4787269 IC50 = 5.0 nM 8.30
CHEMBL4764756 IC50 = 6.0 nM 8.22
CHEMBL4785028 IC50 = 8.0 nM 8.10
CHEMBL4743561 IC50 = 12.0 nM 7.92
CHEMBL4757431 IC50 = 21.0 nM 7.68
CHEMBL4755524 IC50 = 32.0 nM 7.50
CHEMBL4746396 IC50 = 32.0 nM 7.50
CHEMBL4795993 IC50 = 37.0 nM 7.43
CHEMBL4791136 IC50 = 38.0 nM 7.42
CHEMBL4749973 IC50 = 40.0 nM 7.40
CHEMBL4750622 IC50 = 43.0 nM 7.37
CHEMBL4789590 IC50 = 75.0 nM 7.12
CHEMBL4752921 IC50 = 78.0 nM 7.11
CHEMBL4759018 IC50 = 84.0 nM 7.08
CHEMBL4762761 IC50 = 97.0 nM 7.01
CHEMBL4752471 IC50 = 100.0 nM 7.00
CHEMBL4790632 IC50 = 106.0 nM 6.97
CHEMBL4763087 IC50 = 340.0 nM 6.47
CHEMBL4793486 IC50 = 1073.0 nM 5.97
CHEMBL4776712 IC50 = 1112.0 nM 5.95
CHEMBL4793538 IC50 > 2000.0 nM -
CHEMBL4746326 IC50 > 2000.0 nM -
CHEMBL4752505 IC50 > 2000.0 nM -
CHEMBL4765071 IC50 > 2000.0 nM -
CHEMBL4758288 IC50 > 2000.0 nM -
CHEMBL4757267 IC50 > 2000.0 nM -
CHEMBL4763764 IC50 > 2000.0 nM -