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Assay Detail

CHEMBL4738677

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of tubulin polymerization in human HeLa cells assessed as dynamic tyrosinated microtubules content by incubated for 2 hrs by immunofluorescence staining based assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Tubulin (CHEMBL2095182)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Combretastatin A-4 sulfur-containing heterocyclic derivatives: Synthesis, antiproliferative activities and molecular docking studies.
Faouzi A,Arnaud A,Bancet A,Barette C,Preto J,Do CV,Jordheim LP,Bousfiha Z,Nguyen TTB,Verrière M,Farce A,Fauvarque MO,Barret R,Lomberget T
Eur J Med Chem (2021) 215 : 113275 -113275
PubMed 33618157 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.35 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL107 COLCHICINE 4.0 1 6.70
CHEMBL4780261 1 6.28
CHEMBL4785381 1 6.26
CHEMBL4753697 1 6.14
CHEMBL4762961 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL107 COLCHICINE EC50 = 200.0 nM 6.70
CHEMBL4780261 EC50 = 523.0 nM 6.28
CHEMBL4785381 EC50 = 555.0 nM 6.26
CHEMBL4753697 EC50 = 717.0 nM 6.14
CHEMBL4762961 EC50 > 10000.0 nM -