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Assay Detail

CHEMBL4739182

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human p38alpha expressed in Escherichia coli assessed as incoporation of [33]Pi after 60 mins in presence of [gamma-33P]-ATP by microplate scintillation counter method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 14 (CHEMBL260)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of the epithelial-to- mesenchymal inhibitory activity of indazole-derived imidazoles as dual ALK5/p38α MAP inhibitors.
Liu YY,Guo Z,Wang JY,Wang HM,Da Qi J,Ma J,Piao HR,Jin CH,Jin X
Eur J Med Chem (2021) 216 : 113311 -113311
PubMed 33677350 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.16 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4778963 1 8.40
CHEMBL4748823 1 7.89
CHEMBL4779584 1 7.70
CHEMBL4747557 1 7.64
CHEMBL4788508 1 7.64
CHEMBL4794179 1 7.58
CHEMBL4759911 1 7.50
CHEMBL4780141 1 7.28
CHEMBL4756404 1 7.18
CHEMBL4780013 1 7.15
CHEMBL4752915 1 7.02
CHEMBL4784124 1 6.95
CHEMBL4798879 1 6.48
CHEMBL2364611 GALUNISERTIB 2.0 1 6.31
CHEMBL4740767 1 6.25
CHEMBL3260567 VACTOSERTIB 2.0 1 5.53

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4778963 IC50 = 4.0 nM 8.40
CHEMBL4748823 IC50 = 13.0 nM 7.89
CHEMBL4779584 IC50 = 20.0 nM 7.70
CHEMBL4747557 IC50 = 23.0 nM 7.64
CHEMBL4788508 IC50 = 23.0 nM 7.64
CHEMBL4794179 IC50 = 26.0 nM 7.58
CHEMBL4759911 IC50 = 32.0 nM 7.50
CHEMBL4780141 IC50 = 52.0 nM 7.28
CHEMBL4756404 IC50 = 66.0 nM 7.18
CHEMBL4780013 IC50 = 71.0 nM 7.15
CHEMBL4752915 IC50 = 95.0 nM 7.02
CHEMBL4784124 IC50 = 112.0 nM 6.95
CHEMBL4798879 IC50 = 329.0 nM 6.48
CHEMBL2364611 GALUNISERTIB IC50 = 493.0 nM 6.31
CHEMBL4740767 IC50 = 565.0 nM 6.25
CHEMBL3260567 VACTOSERTIB IC50 = 2950.0 nM 5.53