Assay Detail
Binding
CHEMBL4775686
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Binding affinity to biotinylated human TNFalpha trimer (77 to 233 residues) expressed in Escherichia coli BL21(DE3) by SPR analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Development of Orally Efficacious Allosteric Inhibitors of TNFα via Fragment-Based Drug Design.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 11 | 6.04 | 8.89 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4793441 | — | — | 1 | 8.89 |
| CHEMBL4779359 | — | — | 1 | 8.62 |
| CHEMBL4793924 | — | — | 1 | 8.17 |
| CHEMBL4787790 | — | — | 1 | 8.14 |
| CHEMBL4778232 | — | — | 1 | 7.13 |
| CHEMBL4783333 | — | — | 1 | 5.57 |
| CHEMBL4779860 | — | — | 1 | 4.72 |
| CHEMBL4793685 | — | — | 1 | 4.22 |
| CHEMBL4777805 | — | — | 1 | 3.98 |
| CHEMBL347547 | — | — | 1 | 3.52 |
| CHEMBL1507048 | — | — | 1 | 3.49 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4793441 | — | Kd | = | 1.3 | nM | 8.89 |
| CHEMBL4779359 | — | Kd | = | 2.4 | nM | 8.62 |
| CHEMBL4793924 | — | Kd | = | 6.8 | nM | 8.17 |
| CHEMBL4787790 | — | Kd | = | 7.3 | nM | 8.14 |
| CHEMBL4778232 | — | Kd | = | 74.0 | nM | 7.13 |
| CHEMBL4783333 | — | Kd | = | 2700.0 | nM | 5.57 |
| CHEMBL4779860 | — | Kd | = | 19000.0 | nM | 4.72 |
| CHEMBL4793685 | — | Kd | = | 60000.0 | nM | 4.22 |
| CHEMBL4777805 | — | Kd | = | 106000.0 | nM | 3.98 |
| CHEMBL347547 | — | Kd | = | 300000.0 | nM | 3.52 |
| CHEMBL1507048 | — | Kd | = | 325000.0 | nM | 3.49 |