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Assay Detail

CHEMBL4775836

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Covalent inhibition of GRK5 (unknown origin) using porcine brain tubulin as substrate by [gamma32P]ATP based radiometric assay
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G protein-coupled receptor kinase 5 (CHEMBL5678)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Generation of Highly Selective, Potent, and Covalent G Protein-Coupled Receptor Kinase 5 Inhibitors.
Rowlands RA,Chen Q,Bouley RA,Avramova LV,Tesmer JJG,White AD
J Med Chem (2021) 64 : 566 -585
PubMed 33393767 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 6.89 7.72
Kinact 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4786599 2 7.72
CHEMBL4785430 2 6.96
CHEMBL4791260 2 6.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4786599 Ki = 19.0 nM 7.72
CHEMBL4785430 Ki = 110.0 nM 6.96
CHEMBL4791260 Ki = 1000.0 nM 6.00
CHEMBL4791260 Kinact > 1.0 /min -
CHEMBL4785430 Kinact > 1.0 /min -
CHEMBL4786599 Kinact > 0.9 /min -