Assay Detail
Functional
CHEMBL4814800
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at mouse HA-tagged mu opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 15 mins by Glo-sensor reagent based assay
13
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mus musculus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Isolation and Pharmacological Characterization of Six Opioidergic <i>Picralima nitida</i> Alkaloids.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 5.62 | 8.20 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL38874 | DAMGO | — | 2 | 8.20 |
| CHEMBL4871880 | — | — | 2 | 5.60 |
| CHEMBL4856018 | — | — | 2 | 5.50 |
| CHEMBL4846243 | — | — | 2 | 5.30 |
| CHEMBL508955 | AKUAMMICINE | — | 2 | 5.10 |
| CHEMBL4874877 | — | — | 2 | 4.73 |
| CHEMBL4852987 | — | — | 1 | 4.35 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL38874 | DAMGO | IC50 | = | 6.31 | nM | 8.20 |
| CHEMBL38874 | DAMGO | IC50 | = | 6.6 | nM | 8.18 |
| CHEMBL4871880 | — | IC50 | = | 2511.89 | nM | 5.60 |
| CHEMBL4871880 | — | IC50 | = | 2600.0 | nM | 5.58 |
| CHEMBL4856018 | — | IC50 | = | 3140.0 | nM | 5.50 |
| CHEMBL4856018 | — | IC50 | = | 3162.28 | nM | 5.50 |
| CHEMBL4846243 | — | IC50 | = | 5011.87 | nM | 5.30 |
| CHEMBL4846243 | — | IC50 | = | 5240.0 | nM | 5.28 |
| CHEMBL508955 | AKUAMMICINE | IC50 | = | 7943.28 | nM | 5.10 |
| CHEMBL508955 | AKUAMMICINE | IC50 | = | 8240.0 | nM | 5.08 |
| CHEMBL4874877 | — | IC50 | = | 18700.0 | nM | 4.73 |
| CHEMBL4874877 | — | IC50 | = | 19952.62 | nM | 4.70 |
| CHEMBL4852987 | — | IC50 | = | 45000.0 | nM | 4.35 |