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Assay Detail

CHEMBL4815504

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat cortex homogenate acetylcholinesterase using acetylthiocholine iodide as substrate incubated for 20 mins by Ellman's method
39
Total Activities
39
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cortex
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL3199)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Kinetics-Driven Drug Design Strategy for Next-Generation Acetylcholinesterase Inhibitors to Clinical Candidate.
Zhou Y, Fu Y, Yin W, Li J, Wang W, Bai F, Xu S, Gong Q, Peng T, Hong Y, Zhang D, Zhang D, Liu Q, Xu Y, Xu HE, Zhang H, Jiang H, Liu H.
J Med Chem (2021) 64 : 1844 -1855
PubMed 33570950 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 39 7.57 9.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4859647 1 9.07
CHEMBL4846450 1 9.02
CHEMBL502 DONEPEZIL 4.0 1 8.70
CHEMBL4857100 1 8.70
CHEMBL4857791 1 8.57
CHEMBL4853698 1 8.52
CHEMBL4867751 1 8.46
CHEMBL4860655 1 8.45
CHEMBL4856312 1 8.43
CHEMBL4860419 1 8.40
CHEMBL4846622 1 8.36
CHEMBL4867225 1 8.34
CHEMBL4848806 1 8.27
CHEMBL4864695 1 8.23
CHEMBL4851135 1 8.20
CHEMBL4877719 1 8.08
CHEMBL4863392 1 7.93
CHEMBL4864886 1 7.85
CHEMBL4860777 1 7.74
CHEMBL4873794 1 7.65
CHEMBL4854986 1 7.60
CHEMBL4871360 1 7.55
CHEMBL4877366 1 7.35
CHEMBL4851673 1 7.23
CHEMBL4846857 1 7.22
CHEMBL4857091 1 7.18
CHEMBL4868720 1 7.17
CHEMBL4856424 1 7.12
CHEMBL4867651 1 6.79
CHEMBL4869273 1 6.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4859647 IC50 = 0.86 nM 9.07
CHEMBL4846450 IC50 = 0.96 nM 9.02
CHEMBL4857100 IC50 = 2.0 nM 8.70
CHEMBL502 DONEPEZIL IC50 = 2.0 nM 8.70
CHEMBL4857791 IC50 = 2.67 nM 8.57
CHEMBL4853698 IC50 = 3.0 nM 8.52
CHEMBL4867751 IC50 = 3.44 nM 8.46
CHEMBL4860655 IC50 = 3.52 nM 8.45
CHEMBL4856312 IC50 = 3.71 nM 8.43
CHEMBL4860419 IC50 = 4.0 nM 8.40
CHEMBL4846622 IC50 = 4.33 nM 8.36
CHEMBL4867225 IC50 = 4.59 nM 8.34
CHEMBL4848806 IC50 = 5.39 nM 8.27
CHEMBL4864695 IC50 = 5.87 nM 8.23
CHEMBL4851135 IC50 = 6.29 nM 8.20
CHEMBL4877719 IC50 = 8.33 nM 8.08
CHEMBL4863392 IC50 = 11.7 nM 7.93
CHEMBL4864886 IC50 = 14.1 nM 7.85
CHEMBL4860777 IC50 = 18.3 nM 7.74
CHEMBL4873794 IC50 = 22.5 nM 7.65
CHEMBL4854986 IC50 = 25.3 nM 7.60
CHEMBL4871360 IC50 = 28.3 nM 7.55
CHEMBL4877366 IC50 = 44.9 nM 7.35
CHEMBL4851673 IC50 = 59.0 nM 7.23
CHEMBL4846857 IC50 = 60.5 nM 7.22
CHEMBL4857091 IC50 = 66.7 nM 7.18
CHEMBL4868720 IC50 = 67.3 nM 7.17
CHEMBL4856424 IC50 = 75.7 nM 7.12
CHEMBL4867651 IC50 = 163.0 nM 6.79
CHEMBL4869273 IC50 = 176.0 nM 6.75
CHEMBL4876130 IC50 = 181.0 nM 6.74
CHEMBL4877089 IC50 = 226.0 nM 6.65
CHEMBL4863729 IC50 = 260.0 nM 6.58
CHEMBL4856120 IC50 = 306.0 nM 6.51
CHEMBL4871843 IC50 = 348.0 nM 6.46
CHEMBL4850869 IC50 = 404.0 nM 6.39
CHEMBL4860492 IC50 = 646.0 nM 6.19
CHEMBL4869023 IC50 = 1640.0 nM 5.79
CHEMBL4858969 IC50 = 8780.0 nM 5.06