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Assay Detail

CHEMBL4815730

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human NOD2 expressed in HEK-blue cells coexpressing NFkappaB-SEAP reporter assessed as reduction in L18-MDP-induced NF-kappaB activation preincubated for 15 mins followed by L18-MDP stimulation and further incubated for 8 hrs by SEAP reporter gene assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-Blue hNOD2
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design of pyrido[2,3-d]pyrimidin-7-one inhibitors of receptor interacting protein kinase-2 (RIPK2) and nucleotide-binding oligomerization domain (NOD) cell signaling.
Nikhar S, Siokas I, Schlicher L, Lee S, Gyrd-Hansen M, Degterev A, Cuny GD.
Eur J Med Chem (2021) 215 : 113252 -113252
PubMed 33601309 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.14 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4847211 1 8.52
CHEMBL4853179 1 8.40
CHEMBL4870434 1 8.30
CHEMBL4864576 1 8.30
CHEMBL4858463 1 8.05
CHEMBL4859716 1 7.89
CHEMBL136244 1 7.85
CHEMBL136106 1 7.82
CHEMBL4861488 1 7.70
CHEMBL4864600 1 7.66
CHEMBL49120 PD-0166285 1.0 1 7.43
CHEMBL4874031 1 7.03
CHEMBL4868894 1 6.87
CHEMBL4849134 1 6.80
CHEMBL4867716 1 6.66
CHEMBL4871544 1 6.57
CHEMBL4875011 1 6.32
CHEMBL4852513 1 6.29
CHEMBL4853711 1 6.14
CHEMBL4859351 1 6.06
CHEMBL4852860 1 6.02
CHEMBL4847571 1 5.91
CHEMBL4854929 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4847211 IC50 = 3.0 nM 8.52
CHEMBL4853179 IC50 = 4.0 nM 8.40
CHEMBL4870434 IC50 = 5.0 nM 8.30
CHEMBL4864576 IC50 = 5.0 nM 8.30
CHEMBL4858463 IC50 = 9.0 nM 8.05
CHEMBL4859716 IC50 = 13.0 nM 7.89
CHEMBL136244 IC50 = 14.0 nM 7.85
CHEMBL136106 IC50 = 15.0 nM 7.82
CHEMBL4861488 IC50 = 20.0 nM 7.70
CHEMBL4864600 IC50 = 22.0 nM 7.66
CHEMBL49120 PD-0166285 IC50 = 37.0 nM 7.43
CHEMBL4874031 IC50 = 94.0 nM 7.03
CHEMBL4868894 IC50 = 136.0 nM 6.87
CHEMBL4849134 IC50 = 160.0 nM 6.80
CHEMBL4867716 IC50 = 218.0 nM 6.66
CHEMBL4871544 IC50 = 270.0 nM 6.57
CHEMBL4875011 IC50 = 480.0 nM 6.32
CHEMBL4852513 IC50 = 510.0 nM 6.29
CHEMBL4853711 IC50 = 730.0 nM 6.14
CHEMBL4859351 IC50 = 878.0 nM 6.06
CHEMBL4852860 IC50 = 950.0 nM 6.02
CHEMBL4847571 IC50 = 1230.0 nM 5.91
CHEMBL4854929 IC50 = 1980.0 nM 5.70