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Assay Detail

CHEMBL4817610

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His-tagged DCN1 (58 to 259 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay
41
Total Activities
40
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

DCN1-like protein 1 (CHEMBL4105838)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of phenyltriazole thiol-based derivatives as highly potent inhibitors of DCN1-UBC12 interaction.
Zhou W, Xu C, Dong G, Qiao H, Yang J, Liu H, Ding L, Sun K, Zhao W.
Eur J Med Chem (2021) 217 : 113326 -113326
PubMed 33756127 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 6.53 7.58
Ki 1 7.64 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4865016 2 7.64
CHEMBL4440896 1 7.22
CHEMBL4878599 1 7.13
CHEMBL4869654 1 7.11
CHEMBL4847848 1 7.08
CHEMBL4874768 1 6.97
CHEMBL4854235 1 6.94
CHEMBL4851610 1 6.92
CHEMBL1334768 1 6.90
CHEMBL4860176 1 6.87
CHEMBL4868697 1 6.84
CHEMBL4871768 1 6.84
CHEMBL4846458 1 6.81
CHEMBL4847553 1 6.72
CHEMBL4866253 1 6.49
CHEMBL4860279 1 6.42
CHEMBL4856019 1 6.30
CHEMBL4849618 1 6.30
CHEMBL1447470 1 6.30
CHEMBL1448063 1 6.30
CHEMBL85091 1 6.30
CHEMBL4869738 1 6.15
CHEMBL4875837 1 6.15
CHEMBL4866516 1 6.02
CHEMBL1990098 1 5.99
CHEMBL225662 1 5.49
CHEMBL4855199 1 5.38
CHEMBL4857539 1 5.31
CHEMBL4850533 1 -
CHEMBL1370907 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4865016 Ki = 22.92 nM 7.64
CHEMBL4865016 IC50 = 26.36 nM 7.58
CHEMBL4440896 IC50 = 60.28 nM 7.22
CHEMBL4878599 IC50 = 74.69 nM 7.13
CHEMBL4869654 IC50 = 77.55 nM 7.11
CHEMBL4847848 IC50 = 83.91 nM 7.08
CHEMBL4874768 IC50 = 107.56 nM 6.97
CHEMBL4854235 IC50 = 115.0 nM 6.94
CHEMBL4851610 IC50 = 121.21 nM 6.92
CHEMBL1334768 IC50 = 126.99 nM 6.90
CHEMBL4860176 IC50 = 136.1 nM 6.87
CHEMBL4868697 IC50 = 144.74 nM 6.84
CHEMBL4871768 IC50 = 145.99 nM 6.84
CHEMBL4846458 IC50 = 155.8 nM 6.81
CHEMBL4847553 IC50 = 188.53 nM 6.72
CHEMBL4866253 IC50 = 326.46 nM 6.49
CHEMBL4860279 IC50 = 377.57 nM 6.42
CHEMBL85091 IC50 = 500.0 nM 6.30
CHEMBL1448063 IC50 = 500.0 nM 6.30
CHEMBL4849618 IC50 = 500.0 nM 6.30
CHEMBL4856019 IC50 = 500.0 nM 6.30
CHEMBL1447470 IC50 = 500.0 nM 6.30
CHEMBL4869738 IC50 = 706.88 nM 6.15
CHEMBL4875837 IC50 = 710.78 nM 6.15
CHEMBL4866516 IC50 = 957.11 nM 6.02
CHEMBL1990098 IC50 = 1023.29 nM 5.99
CHEMBL225662 IC50 = 3262.67 nM 5.49
CHEMBL4855199 IC50 = 4195.78 nM 5.38
CHEMBL4857539 IC50 = 4871.55 nM 5.31
CHEMBL4867481 IC50 > 10000.0 nM -
CHEMBL4879214 IC50 > 10000.0 nM -
CHEMBL4865774 IC50 > 10000.0 nM -
CHEMBL22794 IC50 > 10000.0 nM -
CHEMBL1334528 IC50 > 10000.0 nM -
CHEMBL4859802 IC50 > 10000.0 nM -
CHEMBL1461372 IC50 > 10000.0 nM -
CHEMBL4872319 IC50 > 10000.0 nM -
CHEMBL3809713 IC50 > 10000.0 nM -
CHEMBL1322981 IC50 > 10000.0 nM -
CHEMBL1370907 IC50 > 10000.0 nM -
CHEMBL4850533 IC50 > 10000.0 nM -