Assay Detail
Binding
CHEMBL4818909
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of BTK in C57Bl/6 mouse splenocyte assessed as reduction in anti-IgM-induced CD69 expression incubated for 1 hr followed anti-IgM stimulation and measured after overnight by flow cytometry analysis
15
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | Splenocyte |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of a Potent and Selective Covalent Inhibitor of Bruton's Tyrosine Kinase with Oral Anti-Inflammatory Activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 7.53 | 8.12 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4852459 | — | — | 1 | 8.12 |
| CHEMBL4846828 | — | — | 1 | 8.10 |
| CHEMBL4851021 | — | — | 1 | 7.96 |
| CHEMBL4875401 | — | — | 1 | 7.89 |
| CHEMBL4853403 | — | — | 2 | 7.72 |
| CHEMBL4854911 | — | — | 1 | 7.70 |
| CHEMBL4871745 | — | — | 1 | 7.52 |
| CHEMBL4871385 | — | — | 1 | 7.38 |
| CHEMBL4849068 | — | — | 1 | 7.10 |
| CHEMBL4861899 | — | — | 1 | 6.89 |
| CHEMBL4873622 | — | — | 1 | - |
| CHEMBL4877611 | — | — | 1 | - |
| CHEMBL4877368 | — | — | 1 | - |
| CHEMBL4863521 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4852459 | — | IC50 | = | 7.6 | nM | 8.12 |
| CHEMBL4846828 | — | IC50 | = | 7.9 | nM | 8.10 |
| CHEMBL4851021 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL4875401 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL4853403 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL4854911 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL4871745 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL4871385 | — | IC50 | = | 42.0 | nM | 7.38 |
| CHEMBL4849068 | — | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL4861899 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL4853403 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL4873622 | — | IC50 | - | — | - | |
| CHEMBL4877611 | — | IC50 | - | — | - | |
| CHEMBL4877368 | — | IC50 | - | — | - | |
| CHEMBL4863521 | — | IC50 | - | — | - |