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Assay Detail

CHEMBL4819608

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Mixed inhibition of human ABCG2 transfected in human HEK293 cells assessed as Ki2 for inhibition of mitoxantrone efflux measured after 30 mins by FACS Calibur cytometric analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Uncompetitive nanomolar dimeric indenoindole inhibitors of the human breast cancer resistance pump ABCG2.
Guragossian N, Belhani B, Moreno A, Nunes MT, Gonzalez-Lobato L, Marminon C, Berthier L, Rocio Andrade Pires AD, Özvegy-Laczka C, Sarkadi B, Terreux R, Bouaziz Z, Berredjem M, Jose J, Di Pietro A, Falson P, Le Borgne M.
Eur J Med Chem (2021) 211 : 113017 -113017
PubMed 33223263 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 6.66 7.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4876781 1 7.38
CHEMBL4875810 1 6.53
CHEMBL3353414 1 6.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4876781 Ki = 42.0 nM 7.38
CHEMBL4875810 Ki = 294.0 nM 6.53
CHEMBL3353414 Ki = 843.0 nM 6.07