Assay Detail
Binding
CHEMBL4819635
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant Escherichia coli LeuRS assessed as inhibition of 14C-radiolabelled leucine transfer to tRNA leucine preincubated for 10 mins followed by addition of ATP and measured after 4 mins by Scintillation counting method
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Leucine--tRNA ligase (CHEMBL4295566) ↗| Type | SINGLE PROTEIN |
| Organism | Escherichia coli (strain K12) |
Publication
Synthesis and structure-activity studies of novel anhydrohexitol-based Leucyl-tRNA synthetase inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 12 | 7.16 | 8.61 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4864835 | — | — | 1 | 8.61 |
| CHEMBL4869028 | — | — | 1 | 8.26 |
| CHEMBL4864965 | — | — | 1 | 7.75 |
| CHEMBL4855635 | — | — | 1 | 7.51 |
| CHEMBL4864716 | — | — | 1 | 7.50 |
| CHEMBL4852287 | — | — | 1 | 7.50 |
| CHEMBL4867227 | — | — | 1 | 7.28 |
| CHEMBL4876966 | — | — | 1 | 6.96 |
| CHEMBL4861722 | — | — | 1 | 6.74 |
| CHEMBL4850408 | — | — | 1 | 6.41 |
| CHEMBL4871282 | — | — | 1 | 5.90 |
| CHEMBL4871079 | — | — | 1 | 5.53 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4864835 | — | Ki | = | 2.48 | nM | 8.61 |
| CHEMBL4869028 | — | Ki | = | 5.51 | nM | 8.26 |
| CHEMBL4864965 | — | Ki | = | 17.83 | nM | 7.75 |
| CHEMBL4855635 | — | Ki | = | 30.94 | nM | 7.51 |
| CHEMBL4864716 | — | Ki | = | 31.62 | nM | 7.50 |
| CHEMBL4852287 | — | Ki | = | 31.28 | nM | 7.50 |
| CHEMBL4867227 | — | Ki | = | 52.36 | nM | 7.28 |
| CHEMBL4876966 | — | Ki | = | 108.35 | nM | 6.96 |
| CHEMBL4861722 | — | Ki | = | 182.35 | nM | 6.74 |
| CHEMBL4850408 | — | Ki | = | 387.66 | nM | 6.41 |
| CHEMBL4871282 | — | Ki | = | 1260.73 | nM | 5.90 |
| CHEMBL4871079 | — | Ki | = | 2969.71 | nM | 5.53 |