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Assay Detail

CHEMBL4821758

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD4-BD1 (unknown origin) preincubated for 15 mins followed by substrate addition and measured after 1 hr
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 3,5-dimethylisoxazole derivatives as novel, potent inhibitors for bromodomain and extraterminal domain (BET) family.
Fang L, Hu Z, Yang Y, Chen P, Zhou J, Zhang H.
Bioorg Med Chem (2021) 39 : 116133 -116133
PubMed 33862375 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.71 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4850099 1 8.52
CHEMBL4873148 1 8.51
CHEMBL4867171 1 8.25
CHEMBL4865930 1 8.06
CHEMBL4863343 1 8.04
CHEMBL4875215 1 7.92
CHEMBL4867208 1 7.89
CHEMBL4860249 1 7.43
CHEMBL1957266 1 7.38
CHEMBL4637198 1 7.28
CHEMBL4859501 1 7.26
CHEMBL4875647 1 7.24
CHEMBL4878278 1 7.13
CHEMBL4857203 1 6.98
CHEMBL4851529 1 -
CHEMBL4877162 1 -
CHEMBL4865463 1 -
CHEMBL4858249 1 -
CHEMBL4848587 1 -
CHEMBL4859291 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4850099 IC50 = 3.0 nM 8.52
CHEMBL4873148 IC50 = 3.1 nM 8.51
CHEMBL4867171 IC50 = 5.6 nM 8.25
CHEMBL4865930 IC50 = 8.8 nM 8.06
CHEMBL4863343 IC50 = 9.2 nM 8.04
CHEMBL4875215 IC50 = 12.0 nM 7.92
CHEMBL4867208 IC50 = 13.0 nM 7.89
CHEMBL4860249 IC50 = 37.0 nM 7.43
CHEMBL1957266 IC50 = 42.0 nM 7.38
CHEMBL4637198 IC50 = 53.0 nM 7.28
CHEMBL4859501 IC50 = 55.0 nM 7.26
CHEMBL4875647 IC50 = 58.0 nM 7.24
CHEMBL4878278 IC50 = 74.0 nM 7.13
CHEMBL4857203 IC50 = 105.0 nM 6.98
CHEMBL4851529 IC50 - -
CHEMBL4877162 IC50 - -
CHEMBL4865463 IC50 - -
CHEMBL4858249 IC50 - -
CHEMBL4848587 IC50 - -
CHEMBL4859291 IC50 - -