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Assay Detail

CHEMBL4822092

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by DAF-FM based fluorescence analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Novel 1,2,4-triazine-quinoline hybrids: The privileged scaffolds as potent multi-target inhibitors of LPS-induced inflammatory response via dual COX-2 and 15-LOX inhibition.
Ghanim AM, Rezq S, Ibrahim TS, Romero DG, Kothayer H.
Eur J Med Chem (2021) 219 : 113457 -113457
PubMed 33892270 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 4.81 5.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4872636 1 5.20
CHEMBL4852008 1 5.17
CHEMBL4861765 1 5.16
CHEMBL4876293 1 5.03
CHEMBL4867950 1 4.89
CHEMBL4853361 1 4.79
CHEMBL118 CELECOXIB 4.0 1 4.71
CHEMBL4876637 1 4.59
CHEMBL4857790 1 4.47
CHEMBL1034 DICLOFENAC SODIUM 4.0 1 4.44
CHEMBL4862389 1 4.43
CHEMBL4862415 1 -
CHEMBL4847404 1 -
CHEMBL4869763 1 -
CHEMBL4856646 1 -
CHEMBL4876862 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4872636 IC50 = 6340.0 nM 5.20
CHEMBL4852008 IC50 = 6760.0 nM 5.17
CHEMBL4861765 IC50 = 6930.0 nM 5.16
CHEMBL4876293 IC50 = 9370.0 nM 5.03
CHEMBL4867950 IC50 = 12750.0 nM 4.89
CHEMBL4853361 IC50 = 16060.0 nM 4.79
CHEMBL118 CELECOXIB IC50 = 19580.0 nM 4.71
CHEMBL4876637 IC50 = 25780.0 nM 4.59
CHEMBL4857790 IC50 = 33490.0 nM 4.47
CHEMBL1034 DICLOFENAC SODIUM IC50 = 36280.0 nM 4.44
CHEMBL4862389 IC50 = 36810.0 nM 4.43
CHEMBL4862415 IC50 > 50000.0 nM -
CHEMBL4847404 IC50 > 50000.0 nM -
CHEMBL4869763 IC50 > 50000.0 nM -
CHEMBL4856646 IC50 > 50000.0 nM -
CHEMBL4876862 IC50 > 50000.0 nM -