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Assay Detail

CHEMBL4822200

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as reduction in 5hmc level incubated for 2 hrs by ELISA
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Methylcytosine dioxygenase TET2 (CHEMBL4523344)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SAR insights into TET2 catalytic domain inhibition: Synthesis of 2-Hydroxy-4-Methylene-pentanedicarboxylates.
Tiwari AD, Guan Y, Grabowski DR, Maciejewski JP, Jha BK, Phillips JG.
Bioorg Med Chem (2021) 39 : 116141 -116141
PubMed 33894507 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 4.79 5.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4851104 1 5.03
CHEMBL4860506 1 4.98
CHEMBL4872110 1 4.96
CHEMBL90852 N-OXALYLGLYCINE 1 4.71
CHEMBL4855677 1 4.26
CHEMBL4870693 1 -
CHEMBL4859276 1 -
CHEMBL4847806 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4851104 IC50 = 9400.0 nM 5.03
CHEMBL4860506 IC50 = 10500.0 nM 4.98
CHEMBL4872110 IC50 = 11000.0 nM 4.96
CHEMBL90852 N-OXALYLGLYCINE IC50 = 19700.0 nM 4.71
CHEMBL4855677 IC50 = 54800.0 nM 4.26
CHEMBL4870693 IC50 = 261500.0 nM -
CHEMBL4859276 IC50 = 108300.0 nM -
CHEMBL4847806 IC50 = 106600.0 nM -