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Assay Detail

CHEMBL4822809

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at ERalpha (unknown origin) expressed in human HEK293T cells assessed as inhibition of 17beta-estradiol-induced transcriptional activities by measuring ERE driven reporter gene expression measured after 24 hrs by dual luciferase reporter gene assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel hybrid conjugates with dual estrogen receptor α degradation and histone deacetylase inhibitory activities for breast cancer therapy.
Zhao C, Tang C, Li C, Ning W, Hu Z, Xin L, Zhou HB, Huang J.
Bioorg Med Chem (2021) 40 : 116185 -116185
PubMed 33965842 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.74 7.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4800654 1 7.38
CHEMBL4875988 1 7.37
CHEMBL4852827 1 5.96
CHEMBL4853571 1 5.90
CHEMBL4851145 1 5.86
CHEMBL4869951 1 5.73
CHEMBL4874175 1 5.68
CHEMBL4849015 1 5.63
CHEMBL4878251 1 5.58
CHEMBL4870293 1 5.50
CHEMBL4873312 1 5.43
CHEMBL4855392 1 5.29
CHEMBL4868001 1 5.28
CHEMBL4849503 1 5.25
CHEMBL4851237 1 5.24
CHEMBL4874242 1 5.23
CHEMBL4859208 1 5.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4800654 IC50 = 42.0 nM 7.38
CHEMBL4875988 IC50 = 43.0 nM 7.37
CHEMBL4852827 IC50 = 1106.0 nM 5.96
CHEMBL4853571 IC50 = 1254.0 nM 5.90
CHEMBL4851145 IC50 = 1396.0 nM 5.86
CHEMBL4869951 IC50 = 1870.0 nM 5.73
CHEMBL4874175 IC50 = 2080.0 nM 5.68
CHEMBL4849015 IC50 = 2320.0 nM 5.63
CHEMBL4878251 IC50 = 2610.0 nM 5.58
CHEMBL4870293 IC50 = 3130.0 nM 5.50
CHEMBL4873312 IC50 = 3680.0 nM 5.43
CHEMBL4855392 IC50 = 5075.0 nM 5.29
CHEMBL4868001 IC50 = 5270.0 nM 5.28
CHEMBL4849503 IC50 = 5670.0 nM 5.25
CHEMBL4851237 IC50 = 5781.0 nM 5.24
CHEMBL4874242 IC50 = 5900.0 nM 5.23
CHEMBL4859208 IC50 = 6120.0 nM 5.21