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Assay Detail

CHEMBL4822910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured by Ellman's method
21
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Serum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL1914)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of garlic-related unsymmetrical thiosulfonates as potential Alzheimer's disease therapeutics: In vitro and in silico study.
Zilbeyaz K, Oztekin A, Kutluana EG.
Bioorg Med Chem (2021) 40 : 116194 -116194
PubMed 33979775 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.86 7.84
Ki 10 6.90 7.76

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL636 RIVASTIGMINE 4.0 1 7.84
CHEMBL4860610 2 7.76
CHEMBL4871975 2 7.42
CHEMBL4868918 2 6.99
CHEMBL4852721 2 6.99
CHEMBL4863431 2 6.70
CHEMBL4858117 2 6.70
CHEMBL4875944 2 6.70
CHEMBL4845755 2 6.68
CHEMBL4867081 2 6.67
CHEMBL1995010 2 6.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL636 RIVASTIGMINE IC50 = 14.3 nM 7.84
CHEMBL4860610 Ki = 17.5 nM 7.76
CHEMBL4871975 Ki = 37.9 nM 7.42
CHEMBL4860610 IC50 = 53.3 nM 7.27
CHEMBL4871975 IC50 = 84.5 nM 7.07
CHEMBL4852721 Ki = 102.1 nM 6.99
CHEMBL4868918 Ki = 102.6 nM 6.99
CHEMBL4868918 IC50 = 117.9 nM 6.93
CHEMBL4852721 IC50 = 159.4 nM 6.80
CHEMBL4863431 Ki = 198.7 nM 6.70
CHEMBL4858117 Ki = 201.2 nM 6.70
CHEMBL4875944 IC50 = 201.5 nM 6.70
CHEMBL4858117 IC50 = 202.5 nM 6.69
CHEMBL4845755 Ki = 207.6 nM 6.68
CHEMBL4867081 IC50 = 214.3 nM 6.67
CHEMBL1995010 Ki = 217.6 nM 6.66
CHEMBL4875944 Ki = 251.5 nM 6.60
CHEMBL4867081 Ki = 302.5 nM 6.52
CHEMBL4845755 IC50 = 302.7 nM 6.52
CHEMBL4863431 IC50 = 314.5 nM 6.50
CHEMBL1995010 IC50 = 318.5 nM 6.50