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Assay Detail

CHEMBL4824010

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Drug metabolism in human HEp-2 cells assessed as 1-NTP metabolite concentration at EC50 over 48 hrs by LC-MS/MS analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEp-2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

ADMET (CHEMBL612558)
Type ADMET

Publication

Prodrugs of a 1'-CN-4-Aza-7,9-dideazaadenosine <i>C</i>-Nucleoside Leading to the Discovery of Remdesivir (GS-5734) as a Potent Inhibitor of Respiratory Syncytial Virus with Efficacy in the African Green Monkey Model of RSV.
Mackman RL, Hui HC, Perron M, Murakami E, Palmiotti C, Lee G, Stray K, Zhang L, Goyal B, Chun K, Byun D, Siegel D, Simonovich S, Du Pont V, Pitts J, Babusis D, Vijjapurapu A, Lu X, Kim C, Zhao X, Chan J, Ma B, Lye D, Vandersteen A, Wortman S, Barrett KT, Toteva M, Jordan R, Subramanian R, Bilello JP, Cihlar T.
J Med Chem (2021) 64 : 5001 -5017
PubMed 33835812 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.71 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4065616 REMDESIVIR 4.0 1 6.96
CHEMBL2016757 GS-441524 1.0 1 6.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4065616 REMDESIVIR IC50 = 110.0 nM 6.96
CHEMBL2016757 GS-441524 IC50 = 350.0 nM 6.46