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Assay Detail

CHEMBL4825142

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human AChE expressed in HEK293 cells by Ellman's method
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Evaluation of γ-carboline-phenothiazine conjugates as simultaneous NMDA receptor blockers and cholinesterase inhibitors.
Schwarthoff S, Tischer N, Sager H, Schätz B, Rohrbach MM, Raztsou I, Robaa D, Gaube F, Arndt HD, Winckler T.
Bioorg Med Chem (2021) 46 : 116355 -116355
PubMed 34391122 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 5.53 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3605361 1 9.48
CHEMBL807 MEMANTINE 4.0 1 8.31
CHEMBL3605356 1 7.10
CHEMBL4855045 1 6.29
CHEMBL4850585 1 6.27
CHEMBL4873748 1 5.96
CHEMBL4849276 1 5.72
CHEMBL4850804 1 5.66
CHEMBL4870561 1 5.66
CHEMBL4848411 1 5.64
CHEMBL4874126 1 5.58
CHEMBL4878924 1 5.28
CHEMBL4864593 1 5.21
CHEMBL4860501 1 5.11
CHEMBL4850464 1 5.07
CHEMBL596112 1 5.06
CHEMBL4865927 1 5.04
CHEMBL4863376 1 4.94
CHEMBL4857977 1 4.83
CHEMBL4859070 1 4.75
CHEMBL1712867 1 4.69
CHEMBL12540 1 4.67
CHEMBL4856643 1 4.46
CHEMBL261855 1 4.45
CHEMBL4846730 1 4.30
CHEMBL4849640 1 4.28
CHEMBL502 DONEPEZIL 4.0 1 -
CHEMBL3605369 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3605361 IC50 = 0.33 nM 9.48
CHEMBL807 MEMANTINE IC50 = 4.9 nM 8.31
CHEMBL3605356 IC50 = 80.0 nM 7.10
CHEMBL4855045 IC50 = 510.0 nM 6.29
CHEMBL4850585 IC50 = 540.0 nM 6.27
CHEMBL4873748 IC50 = 1100.0 nM 5.96
CHEMBL4849276 IC50 = 1900.0 nM 5.72
CHEMBL4850804 IC50 = 2200.0 nM 5.66
CHEMBL4870561 IC50 = 2200.0 nM 5.66
CHEMBL4848411 IC50 = 2300.0 nM 5.64
CHEMBL4874126 IC50 = 2600.0 nM 5.58
CHEMBL4878924 IC50 = 5300.0 nM 5.28
CHEMBL4864593 IC50 = 6200.0 nM 5.21
CHEMBL4860501 IC50 = 7800.0 nM 5.11
CHEMBL4850464 IC50 = 8600.0 nM 5.07
CHEMBL596112 IC50 = 8700.0 nM 5.06
CHEMBL4865927 IC50 = 9100.0 nM 5.04
CHEMBL4863376 IC50 = 11400.0 nM 4.94
CHEMBL4857977 IC50 = 14700.0 nM 4.83
CHEMBL4859070 IC50 = 17600.0 nM 4.75
CHEMBL1712867 IC50 = 20400.0 nM 4.69
CHEMBL12540 IC50 = 21400.0 nM 4.67
CHEMBL4856643 IC50 = 35100.0 nM 4.46
CHEMBL261855 IC50 = 35400.0 nM 4.45
CHEMBL4846730 IC50 = 49600.0 nM 4.30
CHEMBL4849640 IC50 = 52700.0 nM 4.28
CHEMBL502 DONEPEZIL IC50 - -
CHEMBL3605369 IC50 - -