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Assay Detail

CHEMBL4825465

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Staphylococcus aureus DNA gyrase assessed as reduction in DNA supercoiling using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorimetric assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

DNA gyrase (CHEMBL3038482)
Type PROTEIN COMPLEX
Organism Staphylococcus aureus

Publication

Structurally Optimized Potent Dual-Targeting NBTI Antibacterials with an Enhanced Bifurcated Halogen-Bonding Propensity.
Kokot M, Weiss M, Zdovc I, Hrast M, Anderluh M, Minovski N.
ACS Med Chem Lett (2021) 12 : 1478 -1485
PubMed 34527181 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.71 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4879265 1 8.40
CHEMBL4873479 1 8.40
CHEMBL4862136 1 8.22
CHEMBL4868908 1 8.15
CHEMBL4866999 1 8.05
CHEMBL4862951 1 8.05
CHEMBL4864750 1 8.05
CHEMBL4873185 1 7.85
CHEMBL4879088 1 7.85
CHEMBL4858549 1 7.58
CHEMBL4861727 1 7.46
CHEMBL4855172 1 6.91
CHEMBL4874688 1 6.55
CHEMBL3317856 GEPOTIDACIN 3.0 1 6.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4879265 IC50 = 4.0 nM 8.40
CHEMBL4873479 IC50 = 4.0 nM 8.40
CHEMBL4862136 IC50 = 6.0 nM 8.22
CHEMBL4868908 IC50 = 7.0 nM 8.15
CHEMBL4866999 IC50 = 9.0 nM 8.05
CHEMBL4862951 IC50 = 9.0 nM 8.05
CHEMBL4864750 IC50 = 9.0 nM 8.05
CHEMBL4873185 IC50 = 14.0 nM 7.85
CHEMBL4879088 IC50 = 14.0 nM 7.85
CHEMBL4858549 IC50 = 26.0 nM 7.58
CHEMBL4861727 IC50 = 35.0 nM 7.46
CHEMBL4855172 IC50 = 123.0 nM 6.91
CHEMBL4874688 IC50 = 283.0 nM 6.55
CHEMBL3317856 GEPOTIDACIN IC50 = 374.0 nM 6.43