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Assay Detail

CHEMBL4828623

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His-tagged BRD4 BD2 using biotin-labelled histone H4 acetylated peptide as substrate by TR-FRET assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Substituted 2,3-Benzodiazepines Derivatives as Bromodomain BRD4 Inhibitors.
Sabnis RW.
ACS Med Chem Lett (2021) 12 : 1530 -1531
PubMed 34676032 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.91 7.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4851953 1 7.11
CHEMBL4854720 1 6.96
CHEMBL4848064 1 6.93
CHEMBL4864279 1 6.87
CHEMBL4858472 1 6.82
CHEMBL4855093 1 6.78

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4851953 IC50 = 78.4 nM 7.11
CHEMBL4854720 IC50 = 109.0 nM 6.96
CHEMBL4848064 IC50 = 118.0 nM 6.93
CHEMBL4864279 IC50 = 136.0 nM 6.87
CHEMBL4858472 IC50 = 150.0 nM 6.82
CHEMBL4855093 IC50 = 166.0 nM 6.78