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Assay Detail

CHEMBL4829580

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Binding
Inhibition of [3H]DA uptake at VMAT2 in rat striatal synaptosome incubated for 10 mins followed by [3H]DA addition and measured after 20 mins by TopCount scintillation counting method
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Striatum
Subcellular Synaptosome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Synaptic vesicular amine transporter (CHEMBL4828)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and analysis of dihydrotetrabenazine derivatives as novel vesicular monoamine transporter 2 inhibitors.
Yang Y, Yu D, Zhu X, Du G, Wang W, Zou F, Wang H, Zhang R, Ye L, Tian J.
Eur J Med Chem (2021) 224 : 113718 -113718
PubMed 34329999 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.82 8.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4873900 1 8.29
CHEMBL4874168 1 8.24
CHEMBL4854730 1 8.22
CHEMBL117785 TETRABENAZINE 4.0 1 7.96
CHEMBL4859304 1 7.75
CHEMBL576222 DIHYDROTETRABENAZINE -1.0 1 7.70
CHEMBL4860699 1 7.55
CHEMBL4874676 1 7.45
CHEMBL4867332 1 7.19
CHEMBL567232 1 -
CHEMBL4867056 1 -
CHEMBL213814 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4873900 IC50 = 5.14 nM 8.29
CHEMBL4874168 IC50 = 5.72 nM 8.24
CHEMBL4854730 IC50 = 6.04 nM 8.22
CHEMBL117785 TETRABENAZINE IC50 = 10.98 nM 7.96
CHEMBL4859304 IC50 = 17.83 nM 7.75
CHEMBL576222 DIHYDROTETRABENAZINE IC50 = 20.01 nM 7.70
CHEMBL4860699 IC50 = 27.89 nM 7.55
CHEMBL4874676 IC50 = 35.24 nM 7.45
CHEMBL4867332 IC50 = 64.91 nM 7.19
CHEMBL567232 IC50 - -
CHEMBL4867056 IC50 - -
CHEMBL213814 IC50 - -