Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4831022

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NEK2 (unknown origin) using 5' FAM-KKLNRTLSVA-COOH peptide as a substrate in the presence of ATP incubated for 60 mins by microfluidics assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase Nek2 (CHEMBL3835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of imidazo[1,2-a]pyridine-thiophene derivatives as FLT3 and FLT3 mutants inhibitors for acute myeloid leukemia through structure-based optimization of an NEK2 inhibitor.
Zhang L, Lakkaniga NR, Bharate JB, Mcconnell N, Wang X, Kharbanda A, Leung YK, Frett B, Shah NP, Li HY.
Eur J Med Chem (2021) 225 : 113776 -113776
PubMed 34479037 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 5.88 8.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4860697 1 8.49
CHEMBL4863351 1 5.89
CHEMBL4868026 1 5.63
CHEMBL4847680 1 4.70
CHEMBL4870662 1 4.70
CHEMBL4878572 1 -
CHEMBL4868818 1 -
CHEMBL4855716 1 -
CHEMBL4874305 1 -
CHEMBL4872048 1 -
CHEMBL4849272 1 -
CHEMBL4860913 1 -
CHEMBL4849959 1 -
CHEMBL4852822 1 -
CHEMBL4863689 1 -
CHEMBL4876014 1 -
CHEMBL4859133 1 -
CHEMBL4867699 1 -
CHEMBL4872616 1 -
CHEMBL4846528 1 -
CHEMBL4852877 1 -
CHEMBL4859180 1 -
CHEMBL4869856 1 -
CHEMBL4879086 1 -
CHEMBL4857928 1 -
CHEMBL4873041 1 -
CHEMBL4863364 1 -
CHEMBL4878277 1 -
CHEMBL4857926 1 -
CHEMBL4877108 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4860697 IC50 = 3.2 nM 8.49
CHEMBL4863351 IC50 = 1300.0 nM 5.89
CHEMBL4868026 IC50 = 2350.0 nM 5.63
CHEMBL4847680 IC50 = 20000.0 nM 4.70
CHEMBL4870662 IC50 = 20000.0 nM 4.70
CHEMBL4878572 IC50 > 20000.0 nM -
CHEMBL4868818 IC50 > 20000.0 nM -
CHEMBL4855716 IC50 > 20000.0 nM -
CHEMBL4874305 IC50 > 20000.0 nM -
CHEMBL4872048 IC50 > 20000.0 nM -
CHEMBL4849272 IC50 > 20000.0 nM -
CHEMBL4860913 IC50 > 20000.0 nM -
CHEMBL4849959 IC50 > 20000.0 nM -
CHEMBL4852822 IC50 > 20000.0 nM -
CHEMBL4863689 IC50 > 20000.0 nM -
CHEMBL4876014 IC50 > 20000.0 nM -
CHEMBL4859133 IC50 > 20000.0 nM -
CHEMBL4867699 IC50 > 20000.0 nM -
CHEMBL4872616 IC50 > 20000.0 nM -
CHEMBL4846528 IC50 > 20000.0 nM -
CHEMBL4852877 IC50 > 20000.0 nM -
CHEMBL4859180 IC50 > 20000.0 nM -
CHEMBL4869856 IC50 > 20000.0 nM -
CHEMBL4879086 IC50 > 20000.0 nM -
CHEMBL4857928 IC50 > 20000.0 nM -
CHEMBL4873041 IC50 > 20000.0 nM -
CHEMBL4863364 IC50 > 20000.0 nM -
CHEMBL4878277 IC50 > 20000.0 nM -
CHEMBL4857926 IC50 > 20000.0 nM -
CHEMBL4877108 IC50 > 20000.0 nM -