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Assay Detail

CHEMBL4832186

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 10mer-Thr-FAM probe from WDR5 (unknown origin) incubated for 30 mins by fluorescence polarizaton assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

WD repeat-containing protein 5 (CHEMBL1075317)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a potent MLL1 and WDR5 protein-protein interaction inhibitor with in vivo antitumor activity.
Chen W, Chen X, Li D, Wang X, Long G, Jiang Z, You Q, Guo X.
Eur J Med Chem (2021) 223 : 113677 -113677
PubMed 34225179 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.31 8.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4845887 1 8.19
CHEMBL3884329 1 8.12
CHEMBL4861912 1 8.07
CHEMBL4851969 1 8.05
CHEMBL4869658 1 8.05
CHEMBL4852951 1 7.95
CHEMBL4852048 1 7.80
CHEMBL4863061 1 7.80
CHEMBL4852196 1 7.69
CHEMBL4850959 1 7.47
CHEMBL4864032 1 7.39
CHEMBL4847277 1 7.25
CHEMBL4868412 1 7.22
CHEMBL4868706 1 7.17
CHEMBL3884212 1 7.16
CHEMBL4868755 1 7.14
CHEMBL4847340 1 7.03
CHEMBL4857312 1 7.02
CHEMBL4878567 1 7.00
CHEMBL4877455 1 6.90
CHEMBL4852501 1 6.83
CHEMBL4859991 1 6.75
CHEMBL4846292 1 6.41
CHEMBL4853161 1 6.35
CHEMBL4866458 1 5.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4845887 IC50 = 6.5 nM 8.19
CHEMBL3884329 IC50 = 7.6 nM 8.12
CHEMBL4861912 IC50 = 8.6 nM 8.07
CHEMBL4851969 IC50 = 9.0 nM 8.05
CHEMBL4869658 IC50 = 8.9 nM 8.05
CHEMBL4852951 IC50 = 11.2 nM 7.95
CHEMBL4852048 IC50 = 15.8 nM 7.80
CHEMBL4863061 IC50 = 15.7 nM 7.80
CHEMBL4852196 IC50 = 20.4 nM 7.69
CHEMBL4850959 IC50 = 33.9 nM 7.47
CHEMBL4864032 IC50 = 41.0 nM 7.39
CHEMBL4847277 IC50 = 55.6 nM 7.25
CHEMBL4868412 IC50 = 59.7 nM 7.22
CHEMBL4868706 IC50 = 67.8 nM 7.17
CHEMBL3884212 IC50 = 70.0 nM 7.16
CHEMBL4868755 IC50 = 73.0 nM 7.14
CHEMBL4847340 IC50 = 93.5 nM 7.03
CHEMBL4857312 IC50 = 95.3 nM 7.02
CHEMBL4878567 IC50 = 98.8 nM 7.00
CHEMBL4877455 IC50 = 125.0 nM 6.90
CHEMBL4852501 IC50 = 147.9 nM 6.83
CHEMBL4859991 IC50 = 179.4 nM 6.75
CHEMBL4846292 IC50 = 390.2 nM 6.41
CHEMBL4853161 IC50 = 443.8 nM 6.35
CHEMBL4866458 IC50 = 1036.0 nM 5.99