Assay Detail
Binding
CHEMBL4837037
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant SIRT5 assessed as inhibition of substrate desuccinylation using Fluor de Lys-Succinyl green peptide as substrate in presence of NAD+ measured by fluorometric assay
3
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
NAD-dependent protein deacylase sirtuin-5, mitochondrial (CHEMBL2163183) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Identification of isoform/domain-selective fragments from the selection of DNA-encoded dynamic library.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 4.55 | 4.55 |
| Ki | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1140 | NIACINAMIDE | 4.0 | 1 | 4.55 |
| CHEMBL4870282 | — | — | 2 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1140 | NIACINAMIDE | IC50 | = | 28000.0 | nM | 4.55 |
| CHEMBL4870282 | — | Ki | = | 122000.0 | nM | - |
| CHEMBL4870282 | — | IC50 | = | 183000.0 | nM | - |