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Assay Detail

CHEMBL4839364

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 5-TAMRA-labelled Tracer A from human GST-fused/FLAG-tagged full length ATR/human N-terminal FLAG-tagged full length ATRIP expressed in HEK293-6E cells preincubated for 15 mins followed by tracer A addition and measured after 30 mins by TR-FRET based competition binding assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK2936E
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

ATR/ATRIP (CHEMBL4106138)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

BAY-8400: A Novel Potent and Selective DNA-PK Inhibitor which Shows Synergistic Efficacy in Combination with Targeted Alpha Therapies.
Berger M, Wortmann L, Buchgraber P, Lücking U, Zitzmann-Kolbe S, Wengner AM, Bader B, Bömer U, Briem H, Eis K, Rehwinkel H, Bartels F, Moosmayer D, Eberspächer U, Lienau P, Hammer S, Schatz CA, Wang Q, Wang Q, Mumberg D, Nising CF, Siemeister G.
J Med Chem (2021) 64 : 12723 -12737
PubMed 34428039 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 5.88 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4870563 1 7.05
CHEMBL4860637 1 6.93
CHEMBL4860779 1 6.85
CHEMBL4869632 1 6.71
CHEMBL4875626 1 6.57
CHEMBL4868400 1 6.50
CHEMBL4851439 1 6.41
CHEMBL4852502 1 6.39
CHEMBL4849035 1 6.26
CHEMBL4871998 1 6.10
CHEMBL4850640 1 6.04
CHEMBL4855691 1 5.93
CHEMBL4862738 1 5.91
CHEMBL4860129 1 5.88
CHEMBL4877926 1 5.78
CHEMBL4857669 1 5.75
CHEMBL4873263 1 5.67
CHEMBL4863567 1 5.54
CHEMBL4867528 1 5.31
CHEMBL4878956 1 5.24
CHEMBL4864349 1 5.17
CHEMBL4865476 1 4.89
CHEMBL4873491 1 4.77
CHEMBL4851045 1 4.76
CHEMBL4850455 1 4.71
CHEMBL4847559 1 -
CHEMBL4879247 1 -
CHEMBL4868065 1 -
CHEMBL4865490 1 -
CHEMBL4876439 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4870563 IC50 = 90.0 nM 7.05
CHEMBL4860637 IC50 = 117.0 nM 6.93
CHEMBL4860779 IC50 = 141.0 nM 6.85
CHEMBL4869632 IC50 = 197.0 nM 6.71
CHEMBL4875626 IC50 = 270.0 nM 6.57
CHEMBL4868400 IC50 = 314.0 nM 6.50
CHEMBL4851439 IC50 = 394.0 nM 6.41
CHEMBL4852502 IC50 = 407.0 nM 6.39
CHEMBL4849035 IC50 = 551.0 nM 6.26
CHEMBL4871998 IC50 = 790.0 nM 6.10
CHEMBL4850640 IC50 = 907.0 nM 6.04
CHEMBL4855691 IC50 = 1180.0 nM 5.93
CHEMBL4862738 IC50 = 1220.0 nM 5.91
CHEMBL4860129 IC50 = 1320.0 nM 5.88
CHEMBL4877926 IC50 = 1660.0 nM 5.78
CHEMBL4857669 IC50 = 1770.0 nM 5.75
CHEMBL4873263 IC50 = 2160.0 nM 5.67
CHEMBL4863567 IC50 = 2880.0 nM 5.54
CHEMBL4867528 IC50 = 4920.0 nM 5.31
CHEMBL4878956 IC50 = 5750.0 nM 5.24
CHEMBL4864349 IC50 = 6750.0 nM 5.17
CHEMBL4865476 IC50 = 13000.0 nM 4.89
CHEMBL4873491 IC50 = 16900.0 nM 4.77
CHEMBL4851045 IC50 = 17200.0 nM 4.76
CHEMBL4850455 IC50 = 19700.0 nM 4.71
CHEMBL4847559 IC50 > 20000.0 nM -
CHEMBL4879247 IC50 > 20000.0 nM -
CHEMBL4868065 IC50 > 20000.0 nM -
CHEMBL4865490 IC50 > 20000.0 nM -
CHEMBL4876439 IC50 > 10000.0 nM -