Assay Detail
Binding
CHEMBL4840155
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant full length N-terminal GST tagged MEK7 expressed in Sf21 cells using JNK1 as substrate pre-incubated for 30 mins followed by addition of ATP and measured after 150 mins by ADP-Glo kinase assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf21 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Dual specificity mitogen-activated protein kinase kinase 7 (CHEMBL3530) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Rational Design, Optimization, and Biological Evaluation of Novel MEK4 Inhibitors against Pancreatic Adenocarcinoma.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.86 | 7.02 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4866649 | — | — | 1 | 7.02 |
| CHEMBL4879048 | — | — | 1 | 6.27 |
| CHEMBL4872723 | — | — | 1 | 6.08 |
| CHEMBL4865159 | — | — | 1 | 6.04 |
| CHEMBL4871202 | — | — | 1 | 5.89 |
| CHEMBL4875545 | — | — | 1 | 5.89 |
| CHEMBL4875241 | — | — | 1 | 5.57 |
| CHEMBL4875961 | — | — | 1 | 5.54 |
| CHEMBL4871178 | — | — | 1 | 5.19 |
| CHEMBL4871476 | — | — | 1 | 5.06 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4866649 | — | IC50 | = | 96.0 | nM | 7.02 |
| CHEMBL4879048 | — | IC50 | = | 540.0 | nM | 6.27 |
| CHEMBL4872723 | — | IC50 | = | 840.0 | nM | 6.08 |
| CHEMBL4865159 | — | IC50 | = | 920.0 | nM | 6.04 |
| CHEMBL4871202 | — | IC50 | = | 1300.0 | nM | 5.89 |
| CHEMBL4875545 | — | IC50 | = | 1300.0 | nM | 5.89 |
| CHEMBL4875241 | — | IC50 | = | 2700.0 | nM | 5.57 |
| CHEMBL4875961 | — | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL4871178 | — | IC50 | = | 6400.0 | nM | 5.19 |
| CHEMBL4871476 | — | IC50 | = | 8700.0 | nM | 5.06 |