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Assay Detail

CHEMBL4841584

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated with compound for 2 hrs followed by LPS stimulation measured after 18 hrs by gGriess reagent based assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Total Syntheses and Anti-inflammatory Activities of Syringin and Its Natural Analogues.
Dong H, Wu M, Wang Y, Du W, He Y, Shi Z.
J Nat Prod (2021) 84 : 2866 -2874
PubMed 34658231 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 4.86 5.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL118 CELECOXIB 4.0 1 5.64
CHEMBL3236505 1 4.84
CHEMBL228183 1 4.81
CHEMBL4874788 1 4.78
CHEMBL250872 SYRINGIN 1 4.76
CHEMBL3916405 1 4.73
CHEMBL4859245 1 4.69
CHEMBL4846998 1 4.60
CHEMBL2332681 DIHYDROSYRINGIN 1 -
CHEMBL4856262 1 -
CHEMBL4861531 1 -
CHEMBL4878502 1 -
CHEMBL251059 SINAPALDEHYDE GLUCOSIDE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL118 CELECOXIB IC50 = 2300.0 nM 5.64
CHEMBL3236505 IC50 = 14600.0 nM 4.84
CHEMBL228183 IC50 = 15400.0 nM 4.81
CHEMBL4874788 IC50 = 16600.0 nM 4.78
CHEMBL250872 SYRINGIN IC50 = 17200.0 nM 4.76
CHEMBL3916405 IC50 = 18700.0 nM 4.73
CHEMBL4859245 IC50 = 20400.0 nM 4.69
CHEMBL4846998 IC50 = 25100.0 nM 4.60
CHEMBL2332681 DIHYDROSYRINGIN IC50 > 100000.0 nM -
CHEMBL4856262 IC50 > 100000.0 nM -
CHEMBL4861531 IC50 > 100000.0 nM -
CHEMBL4878502 IC50 > 100000.0 nM -
CHEMBL251059 SINAPALDEHYDE GLUCOSIDE IC50 > 100000.0 nM -