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Assay Detail

CHEMBL4843799

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD4 in human whole blood assessed as reduction in LPS-induced IL-6 secretion preincubated for 30 mins followed by LPS stimulation and measured after 24 hrs by MSD assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Novel Bromodomain and Extra Terminal Domain (BET) Protein Inhibitor, I-BET282E, Suitable for Clinical Progression.
Jones KL, Beaumont DM, Bernard SG, Bit RA, Campbell SP, Chung CW, Cutler L, Demont EH, Dennis K, Gordon L, Gray JR, Haase MV, Lewis AJ, McCleary S, Mitchell DJ, Moore SM, Parr N, Robb OJ, Smithers N, Soden PE, Suckling CJ, Taylor S, Walker AL, Watson RJ, Prinjha RK.
J Med Chem (2021) 64 : 12200 -12227
PubMed 34387088 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.39 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4874801 1 6.80
CHEMBL4853472 1 6.70
CHEMBL4874356 1 6.60
CHEMBL4861790 1 6.60
CHEMBL4878439 1 6.50
CHEMBL4875886 1 6.50
CHEMBL4874790 1 6.40
CHEMBL4855373 1 6.40
CHEMBL1232461 MOLIBRESIB 2.0 1 6.20
CHEMBL2017291 1 5.90
CHEMBL4849704 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4874801 IC50 = 158.49 nM 6.80
CHEMBL4853472 IC50 = 199.53 nM 6.70
CHEMBL4874356 IC50 = 251.19 nM 6.60
CHEMBL4861790 IC50 = 251.19 nM 6.60
CHEMBL4878439 IC50 = 316.23 nM 6.50
CHEMBL4875886 IC50 = 316.23 nM 6.50
CHEMBL4874790 IC50 = 398.11 nM 6.40
CHEMBL4855373 IC50 = 398.11 nM 6.40
CHEMBL1232461 MOLIBRESIB IC50 = 630.96 nM 6.20
CHEMBL2017291 IC50 = 1258.93 nM 5.90
CHEMBL4849704 IC50 = 1995.26 nM 5.70