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Assay Detail

CHEMBL4844392

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Protac activity at VHL/AR T878A mutant in human LNCaP cells assessed as induction of AR degradation measured after 24 hrs by western blot analysis
10
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type LNCaP
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

von Hippel-Lindau disease tumor suppressor/Androgen receptor (CHEMBL4523730)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Strategies toward Discovery of Potent and Orally Bioavailable Proteolysis Targeting Chimera Degraders of Androgen Receptor for the Treatment of Prostate Cancer.
Han X, Zhao L, Xiang W, Qin C, Miao B, McEachern D, Wang Y, Metwally H, Wang L, Matvekas A, Wen B, Sun D, Wang S.
J Med Chem (2021) 64 : 12831 -12854
PubMed 34431670 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 8.51 8.85
EC90 5 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4861919 2 8.85
CHEMBL4879244 2 8.85
CHEMBL4867584 2 8.47
CHEMBL4747369 2 8.29
CHEMBL4877348 2 8.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4861919 EC50 = 1.4 nM 8.85
CHEMBL4879244 EC50 = 1.4 nM 8.85
CHEMBL4867584 EC50 = 3.4 nM 8.47
CHEMBL4747369 EC50 = 5.1 nM 8.29
CHEMBL4877348 EC50 = 8.3 nM 8.08
CHEMBL4747369 EC90 = 41.0 nM -
CHEMBL4861919 EC90 = 52.0 nM -
CHEMBL4877348 EC90 = 25.0 nM -
CHEMBL4879244 EC90 = 10.0 nM -
CHEMBL4867584 EC90 = 10.0 nM -