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Assay Detail

CHEMBL4844499

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of purified human plasma kallikrein assessed as inhibition constant using H-D-Pro-Phe-Arg-pNA.2HCl as substrate measured after 3 mins by microplate reader analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Plasma kallikrein (CHEMBL2000)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Berotralstat (BCX7353): Structure-Guided Design of a Potent, Selective, and Oral Plasma Kallikrein Inhibitor to Prevent Attacks of Hereditary Angioedema (HAE).
Kotian PL, Wu M, Vadlakonda S, Chintareddy V, Lu P, Juarez L, Kellogg-Yelder D, Chen X, Muppa S, Chambers-Wilson R, Davis Parker C, Williams J, Polach KJ, Zhang W, Raman K, Babu YS.
J Med Chem (2021) 64 : 12453 -12468
PubMed 34436898 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 9.48 9.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4297502 AVORALSTAT 3.0 1 9.59
CHEMBL5189739 BEROTRALSTAT 4.0 1 9.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4297502 AVORALSTAT Ki = 0.26 nM 9.59
CHEMBL5189739 BEROTRALSTAT Ki = 0.44 nM 9.36