Assay Detail
Binding
CHEMBL4885651
Review assay metadata, readout intent, target linkage, and publication context from the same page.
FLIPR Ca2+ assay (human TRAP1)
2
Total Activities
2
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL6007) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Data for DCP probe BAY-390
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 7.80 | 7.80 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Additional information | Additional information | - | — | Activation with agonist supercinnemaldehyde (SCA), probe added for 10 min, Readout: calcium sensor GCaMP6 (or Fluo8) |
| Target protein type | Target protein type | - | — | TRPA1 overexpressing cell line (e.g. CHO hTRPA1 GCaMP6) |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4514203 | BAY-390 | — | 1 | 7.80 |
| CHEMBL4555711 | BAY-9897 | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4514203 | BAY-390 | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL4555711 | BAY-9897 | IC50 | > | 25000.0 | nM | - |