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Assay Detail

CHEMBL5029285

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kgamma in C5a-stimulated mouse RAW 264 cells assessed as inhibition of AKT phosphorylation at Ser473 residue Pretreated for 15 mins followed by C5a stimulation measured after 3 mins by HTRF assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type RAW 264
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of AZD8154, a Dual PI3Kγδ Inhibitor for the Treatment of Asthma.
Perry MWD, Björhall K, Bold P, Brűlls M, Börjesson U, Carlsson J, Chang HA, Chen Y, Eriksson A, Fihn BM, Fransson R, Fredlund L, Ge H, Huang H, Karabelas K, Lamm Bergström E, Lever S, Lindmark H, Mogemark M, Nikitidis A, Palmgren AP, Pemberton N, Petersen J, Rodrigo Blomqvist M, Smith RW, Thomas MJ, Ullah V, Tyrchan C, Wennberg T, Westin Eriksson A, Yang W, Zhao S, Öster L.
J Med Chem (2021) 64 : 8053 -8075
PubMed 34080862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 8.69 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5091438 1 9.10
CHEMBL4558527 AZD-8154 2.0 1 9.10
CHEMBL5071394 1 9.10
CHEMBL5086461 1 9.00
CHEMBL5077865 1 9.00
CHEMBL5088310 1 9.00
CHEMBL5076543 1 9.00
CHEMBL5088457 1 9.00
CHEMBL5094364 1 9.00
CHEMBL5090799 1 9.00
CHEMBL5089084 1 9.00
CHEMBL5078679 1 8.90
CHEMBL5091128 1 8.90
CHEMBL5081964 1 8.80
CHEMBL5084056 1 8.80
CHEMBL5082066 1 8.80
CHEMBL5094877 1 8.70
CHEMBL5094025 1 8.70
CHEMBL5080551 1 8.60
CHEMBL5087061 1 8.40
CHEMBL5072469 1 8.40
CHEMBL5081634 1 8.30
CHEMBL4129600 1 8.30
CHEMBL5081600 1 8.20
CHEMBL4126156 1 8.10
CHEMBL5082486 1 7.80
CHEMBL5077792 1 7.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5091438 IC50 = 0.7943 nM 9.10
CHEMBL4558527 AZD-8154 IC50 = 0.7943 nM 9.10
CHEMBL5071394 IC50 = 0.7943 nM 9.10
CHEMBL5086461 IC50 = 1.0 nM 9.00
CHEMBL5077865 IC50 = 1.0 nM 9.00
CHEMBL5088310 IC50 = 1.0 nM 9.00
CHEMBL5076543 IC50 = 1.0 nM 9.00
CHEMBL5088457 IC50 = 1.0 nM 9.00
CHEMBL5094364 IC50 = 1.0 nM 9.00
CHEMBL5090799 IC50 = 1.0 nM 9.00
CHEMBL5089084 IC50 = 1.0 nM 9.00
CHEMBL5078679 IC50 = 1.259 nM 8.90
CHEMBL5091128 IC50 = 1.259 nM 8.90
CHEMBL5081964 IC50 = 1.585 nM 8.80
CHEMBL5084056 IC50 = 1.585 nM 8.80
CHEMBL5082066 IC50 = 1.585 nM 8.80
CHEMBL5094877 IC50 = 1.995 nM 8.70
CHEMBL5094025 IC50 = 1.995 nM 8.70
CHEMBL5080551 IC50 = 2.512 nM 8.60
CHEMBL5087061 IC50 = 3.981 nM 8.40
CHEMBL5072469 IC50 = 3.981 nM 8.40
CHEMBL5081634 IC50 = 5.012 nM 8.30
CHEMBL4129600 IC50 = 5.012 nM 8.30
CHEMBL5081600 IC50 = 6.31 nM 8.20
CHEMBL4126156 IC50 = 7.943 nM 8.10
CHEMBL5082486 IC50 = 15.85 nM 7.80
CHEMBL5077792 IC50 = 19.95 nM 7.70