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Assay Detail

CHEMBL5029288

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human PI3Kalpha assessed as reduction in ADP production using Dic8-PIP2 as substrate pre-treated for 15 mins followed by substrate addition measured after 60 mins by ADP-glo assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of AZD8154, a Dual PI3Kγδ Inhibitor for the Treatment of Asthma.
Perry MWD, Björhall K, Bold P, Brűlls M, Börjesson U, Carlsson J, Chang HA, Chen Y, Eriksson A, Fihn BM, Fransson R, Fredlund L, Ge H, Huang H, Karabelas K, Lamm Bergström E, Lever S, Lindmark H, Mogemark M, Nikitidis A, Palmgren AP, Pemberton N, Petersen J, Rodrigo Blomqvist M, Smith RW, Thomas MJ, Ullah V, Tyrchan C, Wennberg T, Westin Eriksson A, Yang W, Zhao S, Öster L.
J Med Chem (2021) 64 : 8053 -8075
PubMed 34080862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.42 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5080551 1 7.80
CHEMBL5086461 1 7.70
CHEMBL5091128 1 7.70
CHEMBL5076543 1 7.70
CHEMBL5094877 1 7.50
CHEMBL5082066 1 7.50
CHEMBL5084056 1 7.50
CHEMBL5072469 1 7.40
CHEMBL5081964 1 7.40
CHEMBL5091438 1 7.40
CHEMBL5088457 1 7.40
CHEMBL5071394 1 7.40
CHEMBL5078679 1 7.40
CHEMBL5094025 1 7.40
CHEMBL5088310 1 7.30
CHEMBL5090799 1 7.30
CHEMBL4558527 AZD-8154 2.0 1 7.20
CHEMBL5077865 1 7.00
CHEMBL5094364 1 6.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5080551 IC50 = 15.85 nM 7.80
CHEMBL5086461 IC50 = 19.95 nM 7.70
CHEMBL5091128 IC50 = 19.95 nM 7.70
CHEMBL5076543 IC50 = 19.95 nM 7.70
CHEMBL5094877 IC50 = 31.62 nM 7.50
CHEMBL5082066 IC50 = 31.62 nM 7.50
CHEMBL5084056 IC50 = 31.62 nM 7.50
CHEMBL5072469 IC50 = 39.81 nM 7.40
CHEMBL5081964 IC50 = 39.81 nM 7.40
CHEMBL5091438 IC50 = 39.81 nM 7.40
CHEMBL5088457 IC50 = 39.81 nM 7.40
CHEMBL5071394 IC50 = 39.81 nM 7.40
CHEMBL5078679 IC50 = 39.81 nM 7.40
CHEMBL5094025 IC50 = 39.81 nM 7.40
CHEMBL5088310 IC50 = 50.12 nM 7.30
CHEMBL5090799 IC50 = 50.12 nM 7.30
CHEMBL4558527 AZD-8154 IC50 = 63.1 nM 7.20
CHEMBL5077865 IC50 = 100.0 nM 7.00
CHEMBL5094364 IC50 = 125.89 nM 6.90