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Assay Detail

CHEMBL5032903

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H] N-alpha methylhistamine from human recombinant histamine H3 receptor expressed in HEK293 cells measured after 90 mins
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Adenosine A<sub>2A</sub>R/A<sub>1</sub>R Antagonists Enabling Additional H<sub>3</sub>R Antagonism for the Treatment of Parkinson's Disease.
Hagenow S, Affini A, Pioli EY, Hinz S, Zhao Y, Porras G, Namasivayam V, Müller CE, Lin JS, Bezard E, Stark H.
J Med Chem (2021) 64 : 8246 -8262
PubMed 34107215 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 8.22 9.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5089580 1 9.55
CHEMBL5093850 1 9.51
CHEMBL5072724 1 9.31
CHEMBL5093935 1 9.22
CHEMBL5094415 1 8.64
CHEMBL5084903 1 8.35
CHEMBL5094263 1 7.96
CHEMBL5088622 1 7.96
CHEMBL2297871 1 7.96
CHEMBL5082920 1 7.77
CHEMBL5081556 1 7.51
CHEMBL5087059 1 7.44
CHEMBL5082650 1 7.28
CHEMBL14638 CIPROXIFAN 1 6.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5089580 Ki = 0.28 nM 9.55
CHEMBL5093850 Ki = 0.31 nM 9.51
CHEMBL5072724 Ki = 0.49 nM 9.31
CHEMBL5093935 Ki = 0.6 nM 9.22
CHEMBL5094415 Ki = 2.3 nM 8.64
CHEMBL5084903 Ki = 4.5 nM 8.35
CHEMBL5094263 Ki = 11.0 nM 7.96
CHEMBL5088622 Ki = 11.0 nM 7.96
CHEMBL2297871 Ki = 11.0 nM 7.96
CHEMBL5082920 Ki = 17.0 nM 7.77
CHEMBL5081556 Ki = 31.0 nM 7.51
CHEMBL5087059 Ki = 36.0 nM 7.44
CHEMBL5082650 Ki = 52.0 nM 7.28
CHEMBL14638 CIPROXIFAN Ki = 224.0 nM 6.65