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Assay Detail

CHEMBL5032967

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PD-1/PD-L1 interaction in human Jurkat cells expressing PD-1/NFAT/Luc cocultured with CHO-K1 cells expressing PD-L1/aAPC assessed as Jurkat T-lymphocyte activation measured after 6 hrs by luciferase reporter gene based luminescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Jurkat
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Syntheses, Biological Evaluations, and Mechanistic Studies of Benzo[<i>c</i>][1,2,5]oxadiazole Derivatives as Potent PD-L1 Inhibitors with <i>In Vivo</i> Antitumor Activity.
Liu L, Yao Z, Wang S, Xie T, Wu G, Zhang H, Zhang P, Wu Y, Yuan H, Sun H.
J Med Chem (2021) 64 : 8391 -8409
PubMed 34115499 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 6.06 6.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5092744 1 6.43
CHEMBL5087877 1 5.68
CHEMBL4089730 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5092744 EC50 = 375.0 nM 6.43
CHEMBL5087877 EC50 = 2075.0 nM 5.68
CHEMBL4089730 EC50 > 10000.0 nM -