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Assay Detail

CHEMBL5035651

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TRKA F589L mutant (unknown origin) preincubated for 30 mins followed by biotinylated TK-peptide substrate addition and measured after 40 mins by FRET-based Z-lyte kinase assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

High affinity nerve growth factor receptor (CHEMBL2815)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Next-Generation Tropomyosin Receptor Kinase Inhibitors for Combating Multiple Resistance Associated with Protein Mutation.
Zhuo LS, Wang MS, Wu FX, Xu HC, Gong Y, Yu ZC, Tian YG, Pang C, Hao GF, Huang W, Yang GF.
J Med Chem (2021) 64 : 15503 -15514
PubMed 34668694 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 8.78 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4298138 REPOTRECTINIB 4.0 1 9.70
CHEMBL5078405 1 9.52
CHEMBL5088000 1 9.52
CHEMBL5092943 1 9.05
CHEMBL4297627 SELITRECTINIB 2.0 1 8.24
CHEMBL5075273 1 8.05
CHEMBL3889654 LAROTRECTINIB 4.0 1 7.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4298138 REPOTRECTINIB IC50 = 0.2 nM 9.70
CHEMBL5078405 IC50 = 0.3 nM 9.52
CHEMBL5088000 IC50 = 0.3 nM 9.52
CHEMBL5092943 IC50 = 0.9 nM 9.05
CHEMBL4297627 SELITRECTINIB IC50 = 5.8 nM 8.24
CHEMBL5075273 IC50 = 9.0 nM 8.05
CHEMBL3889654 LAROTRECTINIB IC50 = 43.1 nM 7.37