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Assay Detail

CHEMBL5035814

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells assessed as reduction in OATP 1B1-mediated [3H]-pitavastatin uptake preincubated for 30 mins followed by [3H]-pitavastatin addition and measured after 2 mins by liquid scintillation analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

A Series of Novel, Highly Potent, and Orally Bioavailable Next-Generation Tricyclic Peptide PCSK9 Inhibitors.
Tucker TJ, Embrey MW, Alleyne C, Amin RP, Bass A, Bhatt B, Bianchi E, Branca D, Bueters T, Buist N, Ha SN, Hafey M, He H, Higgins J, Johns DG, Kerekes AD, Koeplinger KA, Kuethe JT, Li N, Murphy B, Orth P, Salowe S, Shahripour A, Tracy R, Wang W, Wu C, Xiong Y, Zokian HJ, Wood HB, Walji A.
J Med Chem (2021) 64 : 16770 -16800
PubMed 34704436 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 5.98 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5091040 1 6.89
CHEMBL5086475 1 6.22
CHEMBL5081349 1 5.60
CHEMBL5084902 1 5.22
CHEMBL5084416 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5091040 EC50 = 130.0 nM 6.89
CHEMBL5086475 EC50 = 600.0 nM 6.22
CHEMBL5081349 EC50 = 2500.0 nM 5.60
CHEMBL5084902 EC50 = 6000.0 nM 5.22
CHEMBL5084416 EC50 > 22000.0 nM -