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Assay Detail

CHEMBL5037013

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Potentiation of wild-type CFTR (unknown origin) expressed in FRT cells assessed as chloride transport by measuring membrane potential in presence of forskolin
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type FRT
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cystic fibrosis transmembrane conductance regulator (CHEMBL4051)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Icenticaftor (QBW251), a Cystic Fibrosis Transmembrane Conductance Regulator Potentiator with Clinical Efficacy in Cystic Fibrosis and Chronic Obstructive Pulmonary Disease.
Grand DL, Gosling M, Baettig U, Bahra P, Bala K, Brocklehurst C, Budd E, Butler R, Cheung AK, Choudhury H, Collingwood SP, Cox B, Danahay H, Edwards L, Everatt B, Glaenzel U, Glotin AL, Groot-Kormelink P, Hall E, Hatto J, Howsham C, Hughes G, King A, Koehler J, Kulkarni S, Lightfoot M, Nicholls I, Page C, Pergl-Wilson G, Popa MO, Robinson R, Rowlands D, Sharp T, Spendiff M, Stanley E, Steward O, Taylor RJ, Tranter P, Wagner T, Watson H, Williams G, Wright P, Young A, Sandham DA.
J Med Chem (2021) 64 : 7241 -7260
PubMed 34028270 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 6.14 7.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5084140 1 7.37
CHEMBL2010601 IVACAFTOR 4.0 1 7.16
CHEMBL4650318 ICENTICAFTOR 2.0 1 6.95
CHEMBL1949980 1 6.00
CHEMBL44 GENISTEIN 2.0 1 4.85
CHEMBL5080252 1 4.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5084140 EC50 = 43.0 nM 7.37
CHEMBL2010601 IVACAFTOR EC50 = 70.0 nM 7.16
CHEMBL4650318 ICENTICAFTOR EC50 = 113.0 nM 6.95
CHEMBL1949980 EC50 = 990.0 nM 6.00
CHEMBL44 GENISTEIN EC50 = 14200.0 nM 4.85
CHEMBL5080252 EC50 = 30000.0 nM 4.52