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Assay Detail

CHEMBL5037071

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of Cy5-O-Len probe from 6XHis-tagged CRBN/DDB1 (unknown origin) incubated in dark for 1 hr by fluorescence polarization assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Protein cereblon/DNA damage-binding protein 1 (CHEMBL4630734)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Identification of Potent, Selective, and Orally Bioavailable Small-Molecule GSPT1/2 Degraders from a Focused Library of Cereblon Modulators.
Nishiguchi G, Keramatnia F, Min J, Chang Y, Jonchere B, Das S, Actis M, Price J, Chepyala D, Young B, McGowan K, Slavish PJ, Mayasundari A, Jarusiewicz JA, Yang L, Li Y, Fu X, Garrett SH, Papizan JB, Kodali K, Peng J, Pruett Miller SM, Roussel MF, Mullighan C, Fischer M, Rankovic Z.
J Med Chem (2021) 64 : 7296 -7311
PubMed 34042448 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.41 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5084281 1 9.00
CHEMBL5078971 1 8.52
CHEMBL5078004 1 8.05
CHEMBL5079426 1 8.00
CHEMBL5073414 1 7.96
CHEMBL5094191 1 7.82
CHEMBL5084741 1 7.80
CHEMBL4170596 1 7.75
CHEMBL5074427 1 7.64
CHEMBL848 LENALIDOMIDE 4.0 1 6.54
CHEMBL5069911 1 6.47
CHEMBL5090015 1 6.21
CHEMBL4742712 1 6.08
CHEMBL468 THALIDOMIDE 4.0 1 5.86

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5084281 IC50 = 1.0 nM 9.00
CHEMBL5078971 IC50 = 3.0 nM 8.52
CHEMBL5078004 IC50 = 9.0 nM 8.05
CHEMBL5079426 IC50 = 10.0 nM 8.00
CHEMBL5073414 IC50 = 11.0 nM 7.96
CHEMBL5094191 IC50 = 15.0 nM 7.82
CHEMBL5084741 IC50 = 16.0 nM 7.80
CHEMBL4170596 IC50 = 18.0 nM 7.75
CHEMBL5074427 IC50 = 23.0 nM 7.64
CHEMBL848 LENALIDOMIDE IC50 = 286.0 nM 6.54
CHEMBL5069911 IC50 = 337.0 nM 6.47
CHEMBL5090015 IC50 = 612.0 nM 6.21
CHEMBL4742712 IC50 = 824.0 nM 6.08
CHEMBL468 THALIDOMIDE IC50 = 1380.0 nM 5.86