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Assay Detail

CHEMBL5037316

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human partial length BRD4 BD2 expressed in bacterial expression system assessed as dissociation constant by BROMOscan assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

4-Methyl-1,2,3-Triazoles as <i>N</i>-Acetyl-Lysine Mimics Afford Potent BET Bromodomain Inhibitors with Improved Selectivity.
Cui H, Carlson AS, Schleiff MA, Divakaran A, Johnson JA, Buchholz CR, Zahid H, Vail NR, Shi K, Aihara H, Harki DA, Miller GP, Topczewski JJ, Pomerantz WCK.
J Med Chem (2021) 64 : 10497 -10511
PubMed 34236185 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 5 8.42 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3770724 1 9.52
CHEMBL4639128 GSK778 1 9.20
CHEMBL4297454 ABBV-744 1.0 1 8.68
CHEMBL5080584 1 7.55
CHEMBL5083660 1 7.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3770724 Kd = 0.3 nM 9.52
CHEMBL4639128 GSK778 Kd = 0.63 nM 9.20
CHEMBL4297454 ABBV-744 Kd = 2.1 nM 8.68
CHEMBL5080584 Kd = 28.0 nM 7.55
CHEMBL5083660 Kd = 67.0 nM 7.17