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Assay Detail

CHEMBL5039191

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Partial agonist activity at human A2A receptor expressed in HEK293 cell membranes assessed as intrinsic activity by measuring cell index change within 60 mins by Label-Free Impedance-Based Assay
7
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Crystal Structure and Subsequent Ligand Design of a Nonriboside Partial Agonist Bound to the Adenosine A<sub>2A</sub> Receptor.
Amelia T, van Veldhoven JPD, Falsini M, Liu R, Heitman LH, van Westen GJP, Segala E, Verdon G, Cheng RKY, Cooke RM, van der Es D, IJzerman AP.
J Med Chem (2021) 64 : 3827 -3842
PubMed 33764785 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 7.11 8.85
pEC50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL331372 CGS-21680 1 8.85
CHEMBL124345 1 8.01
CHEMBL5093654 1 7.35
CHEMBL5078507 1 6.57
CHEMBL5091876 1 6.09
CHEMBL5088305 1 5.78
CHEMBL5093743 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL331372 CGS-21680 EC50 = 1.413 nM 8.85
CHEMBL124345 EC50 = 9.772 nM 8.01
CHEMBL5093654 EC50 = 44.67 nM 7.35
CHEMBL5078507 EC50 = 269.15 nM 6.57
CHEMBL5091876 EC50 = 812.83 nM 6.09
CHEMBL5088305 EC50 = 1659.59 nM 5.78
CHEMBL5093743 pEC50 - -